A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes

Rodrigo R. Domingues; Gabriel Okuda; Andrea Bernard; Alberto F. Amorim; Juliana De Biasi; Alexandre W. S. Hilsdorf

doi:10.1007/s12686-015-0489-4

A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes

Rodrigo R. Domingues
, Gabriel Okuda
, Andrea Bernard
, Alberto F. Amorim
, Juliana De Biasi
, Alexandre W. S. Hilsdorf

Department of Marine and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes ( Taq I and Hae III) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.

Original language	American English
Pages (from-to)	807-809
Number of pages	3
Journal	Conservation Genetic Resources
Volume	7
Issue number	4
DOIs	https://doi.org/10.1007/s12686-015-0489-4
State	Published - Dec 1 2015

Keywords

COI
Fisheries management
Fishes
Genetics
Georgii
Istiophoridae
Marlin
PCR-RFLP
Pelagic fish
Perspective
Program
Roundscale spearfish
PCR–RFLP

Disciplines

Marine Biology
Oceanography and Atmospheric Sciences and Meteorology

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10.1007/s12686-015-0489-4License: Unspecified

Cite this

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title = "A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes",

abstract = " Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes ( Taq I and Hae III) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.",

keywords = "COI, Fisheries management, Fishes, Genetics, Georgii, Istiophoridae, Marlin, PCR-RFLP, Pelagic fish, Perspective, Program, Roundscale spearfish, PCR–RFLP",

author = "Domingues, \{Rodrigo R.\} and Gabriel Okuda and Andrea Bernard and Amorim, \{Alberto F.\} and \{De Biasi\}, Juliana and Hilsdorf, \{Alexandre W. S.\}",

year = "2015",

month = dec,

day = "1",

doi = "10.1007/s12686-015-0489-4",

language = "American English",

volume = "7",

pages = "807--809",

journal = "Conservation Genetic Resources",

issn = "1877-7260",

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TY - JOUR

T1 - A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes

AU - Domingues, Rodrigo R.

AU - Okuda, Gabriel

AU - Bernard, Andrea

AU - Amorim, Alberto F.

AU - De Biasi, Juliana

AU - Hilsdorf, Alexandre W. S.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes ( Taq I and Hae III) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.

AB - Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes ( Taq I and Hae III) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.

KW - COI

KW - Fisheries management

KW - Fishes

KW - Genetics

KW - Georgii

KW - Istiophoridae

KW - Marlin

KW - PCR-RFLP

KW - Pelagic fish

KW - Perspective

KW - Program

KW - Roundscale spearfish

KW - PCR–RFLP

UR - https://nsuworks.nova.edu/occ_facarticles/744

U2 - 10.1007/s12686-015-0489-4

DO - 10.1007/s12686-015-0489-4

M3 - Article

SN - 1877-7260

VL - 7

SP - 807

EP - 809

JO - Conservation Genetic Resources

JF - Conservation Genetic Resources

IS - 4

ER -

A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes

Abstract

Keywords

Disciplines

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