A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Catherine Chiafair; Dimitri Giarikos; Reza Razeghifard

A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Catherine Chiafair
, Dimitri Giarikos
, Reza Razeghifard

Research output: Contribution to conference › Poster

Abstract

A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.

Original language	American English
State	Published - Mar 17 2014
Event	Spring Meeting of the American Chemical Society (ACS): Chemistry & Materials for Energy - Dallas, United States Duration: Mar 16 2014 → Mar 20 2014

Conference

Conference	Spring Meeting of the American Chemical Society (ACS)
Country/Territory	United States
City	Dallas
Period	3/16/14 → 3/20/14

Disciplines

Physical Sciences and Mathematics

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http://acselb-529643017.us-west-2.elb.amazonaws.com/chem/247nm/program/view.php?obj_id=242739&terms=

Cite this

@conference{7776f3b578104677ad7d2de5af010040,

title = "A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories",

abstract = " A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.",

author = "Catherine Chiafair and Dimitri Giarikos and Reza Razeghifard",

year = "2014",

month = mar,

day = "17",

language = "American English",

note = "Spring Meeting of the American Chemical Society (ACS) : Chemistry \& Materials for Energy ; Conference date: 16-03-2014 Through 20-03-2014",

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TY - CONF

T1 - A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

AU - Chiafair, Catherine

AU - Giarikos, Dimitri

AU - Razeghifard, Reza

PY - 2014/3/17

Y1 - 2014/3/17

N2 - A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.

AB - A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.

UR - https://nsuworks.nova.edu/cnso_chemphys_facpres/1

M3 - Poster

T2 - Spring Meeting of the American Chemical Society (ACS)

Y2 - 16 March 2014 through 20 March 2014

ER -

A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Abstract

Conference

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