A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Reza Razeghifard; Catherine Chiafair; Dimitri Giarikos

doi:10.1021/ed400425d

A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Reza Razeghifard
, Catherine Chiafair
, Dimitri Giarikos

Research output: Contribution to journal › Article › peer-review

Abstract

A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work. © 2013 The American Chemical Society and Division of Chemical Education, Inc.

Original language	American English
Pages (from-to)	141-144
Number of pages	4
Journal	Journal of Chemical Education
Volume	91
Issue number	1
DOIs	https://doi.org/10.1021/ed400425d
State	Published - Jan 14 2014

ASJC Scopus Subject Areas

General Chemistry
Education

Keywords

Upper-Division Undergraduate
Biochemistry
Laboratory Instruction
Collaborative/Cooperative Learning
Bioorganic Chemistry
Qualitative Analysis
Enzymes
Gas Chromatography
Mass Spectrometry
Quantitative Analysis

Disciplines

Biochemistry

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10.1021/ed400425d

Cite this

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title = "A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories",

abstract = "A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work. {\textcopyright} 2013 The American Chemical Society and Division of Chemical Education, Inc.",

keywords = "Upper-Division Undergraduate, Biochemistry, Laboratory Instruction, Collaborative/Cooperative Learning, Bioorganic Chemistry, Qualitative Analysis, Enzymes, Gas Chromatography, Mass Spectrometry, Quantitative Analysis",

author = "Reza Razeghifard and Catherine Chiafair and Dimitri Giarikos",

year = "2014",

month = jan,

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doi = "10.1021/ed400425d",

language = "American English",

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journal = "Journal of Chemical Education",

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TY - JOUR

T1 - A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

AU - Razeghifard, Reza

AU - Chiafair, Catherine

AU - Giarikos, Dimitri

PY - 2014/1/14

Y1 - 2014/1/14

N2 - A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work. © 2013 The American Chemical Society and Division of Chemical Education, Inc.

AB - A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work. © 2013 The American Chemical Society and Division of Chemical Education, Inc.

KW - Upper-Division Undergraduate

KW - Biochemistry

KW - Laboratory Instruction

KW - Collaborative/Cooperative Learning

KW - Bioorganic Chemistry

KW - Qualitative Analysis

KW - Enzymes

KW - Gas Chromatography

KW - Mass Spectrometry

KW - Quantitative Analysis

UR - https://nsuworks.nova.edu/cnso_chemphys_facarticles/71

UR - https://www.scopus.com/pages/publications/84892622756

UR - https://www.scopus.com/pages/publications/84892622756#tab=citedBy

U2 - 10.1021/ed400425d

DO - 10.1021/ed400425d

M3 - Article

SN - 0021-9584

VL - 91

SP - 141

EP - 144

JO - Journal of Chemical Education

JF - Journal of Chemical Education

IS - 1

ER -

A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Abstract

ASJC Scopus Subject Areas

Keywords

Disciplines

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