Angiotensin-II Actions and AT1-Receptors During Statin Withdrawal

Emily Zollner; Ana M Castejon; Antonio G. Tristano; Luigi Cubeddu

Angiotensin-II Actions and AT1-Receptors During Statin Withdrawal

Emily Zollner
, Ana M Castejon
, Antonio G. Tristano
, Luigi Cubeddu

Department of Pharmaceutical Sciences

Research output: Contribution to conference › Presentation

Abstract

Background. Acute discontinuation of statins induces vascular dysfunction and increases cardiovascular events. The mechanisms underlying these events are under investigation. Angiotensin II (AII)-signaling is increased after acute statin withdrawal. Objectives. To investigate whether AII-AT1-receptor expression (AT1-R mRNA) and receptor protein (AT1-R) levels mediate the increase in AII signaling observed after statin withdrawal. Methods and Results. In rat aortic vascular smooth muscle cells (VSMC), simvastatin (0.3-3 μM for 24 hours) inhibited in a concentration-dependent manner AII-stimulated phosphorylation of extracellular-signal regulated kinase 1/2 ERK1/2 (-67+5% with 3 μM; P<0.001), decreased AT1-R mRNA (- 34+ 8 % with 3 μM; P<0.01) and AT1-R protein (- 32+6 % with 3 μM; P<0.01). Removal of simvastatin, led to a rebound increase in mRNA-AT1-R (+39+2 %,

Original language	American English
State	Published - 2008
Event	Health Professions Division Research Day - Nova Southeastern University, Davie Duration: Apr 25 2008 → May 25 2008

Conference

Conference	Health Professions Division Research Day
City	Davie
Period	4/25/08 → 5/25/08

Disciplines

Medicine and Health Sciences

Cite this

@conference{e23490da77e749f98f1b88d9592743cb,

title = "Angiotensin-II Actions and AT1-Receptors During Statin Withdrawal",

abstract = "Background. Acute discontinuation of statins induces vascular dysfunction and increases cardiovascular events. The mechanisms underlying these events are under investigation. Angiotensin II (AII)-signaling is increased after acute statin withdrawal. Objectives. To investigate whether AII-AT1-receptor expression (AT1-R mRNA) and receptor protein (AT1-R) levels mediate the increase in AII signaling observed after statin withdrawal. Methods and Results. In rat aortic vascular smooth muscle cells (VSMC), simvastatin (0.3-3 μM for 24 hours) inhibited in a concentration-dependent manner AII-stimulated phosphorylation of extracellular-signal regulated kinase 1/2 ERK1/2 (-67+5\% with 3 μM; P<0.001), decreased AT1-R mRNA (- 34+ 8 \% with 3 μM; P<0.01) and AT1-R protein (- 32+6 \% with 3 μM; P<0.01). Removal of simvastatin, led to a rebound increase in mRNA-AT1-R (+39+2 \%,",

author = "Emily Zollner and Castejon, \{Ana M\} and Tristano, \{Antonio G.\} and Luigi Cubeddu",

year = "2008",

language = "American English",

note = " Health Professions Division Research Day ; Conference date: 25-04-2008 Through 25-05-2008",

}

TY - CONF

T1 - Angiotensin-II Actions and AT1-Receptors During Statin Withdrawal

AU - Zollner, Emily

AU - Castejon, Ana M

AU - Tristano, Antonio G.

AU - Cubeddu, Luigi

PY - 2008

Y1 - 2008

N2 - Background. Acute discontinuation of statins induces vascular dysfunction and increases cardiovascular events. The mechanisms underlying these events are under investigation. Angiotensin II (AII)-signaling is increased after acute statin withdrawal. Objectives. To investigate whether AII-AT1-receptor expression (AT1-R mRNA) and receptor protein (AT1-R) levels mediate the increase in AII signaling observed after statin withdrawal. Methods and Results. In rat aortic vascular smooth muscle cells (VSMC), simvastatin (0.3-3 μM for 24 hours) inhibited in a concentration-dependent manner AII-stimulated phosphorylation of extracellular-signal regulated kinase 1/2 ERK1/2 (-67+5% with 3 μM; P<0.001), decreased AT1-R mRNA (- 34+ 8 % with 3 μM; P<0.01) and AT1-R protein (- 32+6 % with 3 μM; P<0.01). Removal of simvastatin, led to a rebound increase in mRNA-AT1-R (+39+2 %,

AB - Background. Acute discontinuation of statins induces vascular dysfunction and increases cardiovascular events. The mechanisms underlying these events are under investigation. Angiotensin II (AII)-signaling is increased after acute statin withdrawal. Objectives. To investigate whether AII-AT1-receptor expression (AT1-R mRNA) and receptor protein (AT1-R) levels mediate the increase in AII signaling observed after statin withdrawal. Methods and Results. In rat aortic vascular smooth muscle cells (VSMC), simvastatin (0.3-3 μM for 24 hours) inhibited in a concentration-dependent manner AII-stimulated phosphorylation of extracellular-signal regulated kinase 1/2 ERK1/2 (-67+5% with 3 μM; P<0.001), decreased AT1-R mRNA (- 34+ 8 % with 3 μM; P<0.01) and AT1-R protein (- 32+6 % with 3 μM; P<0.01). Removal of simvastatin, led to a rebound increase in mRNA-AT1-R (+39+2 %,

UR - https://nsuworks.nova.edu/hpdrd/2008/events/88/

M3 - Presentation

T2 - Health Professions Division Research Day

Y2 - 25 April 2008 through 25 May 2008

ER -

Angiotensin-II Actions and AT1-Receptors During Statin Withdrawal

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