Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

Bruce McCord; Quentin Gauthier; Hussain Alghanim; Joana Antunes; Nicole Fernandez Tejero; George Duncan; Kuppareddi Balamurugan

doi:10.1016/j.fsigss.2019.10.061

Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

Bruce McCord
, Quentin Gauthier
, Hussain Alghanim
, Joana Antunes
, Nicole Fernandez Tejero
, George Duncan
, Kuppareddi Balamurugan

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. The first application involves the development of a multiplex amplification capable of simultaneous analysis of 4 different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. We have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status.

Original language	English
Pages (from-to)	485-487
Number of pages	3
Journal	Forensic Science International: Genetics Supplement Series
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/j.fsigss.2019.10.061
State	Published - Dec 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.

ASJC Scopus Subject Areas

Pathology and Forensic Medicine
Genetics

Keywords

Epigenetic
High resolution melt
Pyrosequencing
Serology
Smoking status

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10.1016/j.fsigss.2019.10.061

Cite this

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title = "Applications of epigenetic methylation in body fluid identification, age determination and phenotyping",

abstract = "In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. The first application involves the development of a multiplex amplification capable of simultaneous analysis of 4 different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. We have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status.",

keywords = "Epigenetic, High resolution melt, Pyrosequencing, Serology, Smoking status",

author = "Bruce McCord and Quentin Gauthier and Hussain Alghanim and Joana Antunes and \{Fernandez Tejero\}, Nicole and George Duncan and Kuppareddi Balamurugan",

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year = "2019",

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doi = "10.1016/j.fsigss.2019.10.061",

language = "English",

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T1 - Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

AU - McCord, Bruce

AU - Gauthier, Quentin

AU - Alghanim, Hussain

AU - Antunes, Joana

AU - Fernandez Tejero, Nicole

AU - Duncan, George

AU - Balamurugan, Kuppareddi

PY - 2019/12

Y1 - 2019/12

N2 - In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. The first application involves the development of a multiplex amplification capable of simultaneous analysis of 4 different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. We have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status.

AB - In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. The first application involves the development of a multiplex amplification capable of simultaneous analysis of 4 different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. We have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status.

KW - Epigenetic

KW - High resolution melt

KW - Pyrosequencing

KW - Serology

KW - Smoking status

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UR - https://www.scopus.com/pages/publications/85073749088#tab=citedBy

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DO - 10.1016/j.fsigss.2019.10.061

M3 - Article

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VL - 7

SP - 485

EP - 487

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

IS - 1

ER -

Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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