Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy

Sajjad Alimohammadvand; Masoumeh Kaveh Zenjanab; Parvin Samadi Pakchin; Elaheh Dalir Abdolahinia; Jaleh Barar; Yadollah Omidi; Mohammad M. Pourseif; Marziyeh Fathi; Jalal Shayegh

doi:10.1186/s12896-024-00939-1

Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy

Sajjad Alimohammadvand
, Masoumeh Kaveh Zenjanab
, Parvin Samadi Pakchin
, Elaheh Dalir Abdolahinia
, Jaleh Barar
, Yadollah Omidi
, Mohammad M. Pourseif
, Marziyeh Fathi
, Jalal Shayegh

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: Breast cancer, a formidable global health challenge for women, necessitates innovative therapeutic strategies with enhanced efficacy and minimal side effects. Aripiprazole (ARI), a widely used schizophrenia medication, exhibits promising potential in the treatment of breast cancer. As cancer therapy evolves towards a combination approach, multimodal nano-based delivery systems, such as ARI-loaded niosomes (NIOs) combined with Chitosan-Au nanoparticles for chemo-photothermal therapy, show promise over traditional chemotherapy alone by enhancing targeted efficacy and minimizing side effects. Methods: In this study, a niosomal formulation was designed, incorporating ARI and chitosan-coated AuNPs (i.e. NIOs/AuNPs-CS/ARI), to study the synergistic effect of photothermal/chemotherapy in breast cancer cells. Results: The nanosystems were characterized using UV-Vis spectroscopy and Fourier-transform infrared spectroscopy (FT-IR), confirming the successful synthesis steps. The hydrodynamic diameter of NIOs/AuNPs-CS was determined to be 44.62 nm with a zeta potential of -0.836. Also, Transmission Electron Microscopy (TEM) and Field-Emission Scanning Electron Microscopical (FE-SEM) analysis were performed to assess the size and morphology of NPs. The loading efficiency of ARI in NIOs and NIOs/AuNPs–CS was 75% and 88%, respectively. Furthermore, the release rate of the drug from NIOs/AuNPs–CS is higher than blank NIOs at two pH values (5.8 and 7.4). The cellular uptake of AuNPs-CS-encapsulated NIOs was considerably higher than that of blank NIOs. The Annexin V/PI staining assay showed that the apoptosis/necrosis rate was high in NIOs/AuNPs-CS/ARI (46%) and NIOs/ARI (36%) in 48 h. The results of MTT assessments demonstrated higher cytotoxicity by ARI-loaded NPs. The viability of MCF-7 cells treated with NIOs/AuNPs-CS/ARI was reduced from 60% and 50% to 40% and 20%, respectively, after 24 and 48 h upon laser irradiation. Conclusion: The results of this experiment demonstrated the remarkable effectiveness of NIOs/AuNPs-CS/ARI in cancer treatment, owing to their unique properties, including the PTT capability and pH sensitivity.

Original language	English
Article number	108
Pages (from-to)	108
Journal	BMC Biotechnology
Volume	24
Issue number	1
DOIs	https://doi.org/10.1186/s12896-024-00939-1
State	Published - Dec 24 2024

Bibliographical note

Funding

This study, as part of a DVM thesis, was supported by the Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences (Grant#68893).

Funders	Funder number
Tabriz University	68893

ASJC Scopus Subject Areas

Biotechnology

Keywords

Aripiprazole
Breast cancer
Chitosan
Combination therapy
Gold nanoparticles
Niosomes
Photothermal therapy
Humans
Metal Nanoparticles/chemistry
Liposomes/chemistry
Cell Survival/drug effects
Antineoplastic Agents/chemistry
Gold/chemistry
MCF-7 Cells
Cell Line, Tumor
Female
Aripiprazole/chemistry
Chitosan/chemistry
Breast Neoplasms/drug therapy

Disciplines

Biotechnology

Access to Document

10.1186/s12896-024-00939-1License: CC BY-NC-ND

Cite this

@article{b486b7aa0d3f4da69c1ca223c911de52,

title = "Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy",

abstract = "Introduction: Breast cancer, a formidable global health challenge for women, necessitates innovative therapeutic strategies with enhanced efficacy and minimal side effects. Aripiprazole (ARI), a widely used schizophrenia medication, exhibits promising potential in the treatment of breast cancer. As cancer therapy evolves towards a combination approach, multimodal nano-based delivery systems, such as ARI-loaded niosomes (NIOs) combined with Chitosan-Au nanoparticles for chemo-photothermal therapy, show promise over traditional chemotherapy alone by enhancing targeted efficacy and minimizing side effects. Methods: In this study, a niosomal formulation was designed, incorporating ARI and chitosan-coated AuNPs (i.e. NIOs/AuNPs-CS/ARI), to study the synergistic effect of photothermal/chemotherapy in breast cancer cells. Results: The nanosystems were characterized using UV-Vis spectroscopy and Fourier-transform infrared spectroscopy (FT-IR), confirming the successful synthesis steps. The hydrodynamic diameter of NIOs/AuNPs-CS was determined to be 44.62 nm with a zeta potential of -0.836. Also, Transmission Electron Microscopy (TEM) and Field-Emission Scanning Electron Microscopical (FE-SEM) analysis were performed to assess the size and morphology of NPs. The loading efficiency of ARI in NIOs and NIOs/AuNPs–CS was 75\% and 88\%, respectively. Furthermore, the release rate of the drug from NIOs/AuNPs–CS is higher than blank NIOs at two pH values (5.8 and 7.4). The cellular uptake of AuNPs-CS-encapsulated NIOs was considerably higher than that of blank NIOs. The Annexin V/PI staining assay showed that the apoptosis/necrosis rate was high in NIOs/AuNPs-CS/ARI (46\%) and NIOs/ARI (36\%) in 48 h. The results of MTT assessments demonstrated higher cytotoxicity by ARI-loaded NPs. The viability of MCF-7 cells treated with NIOs/AuNPs-CS/ARI was reduced from 60\% and 50\% to 40\% and 20\%, respectively, after 24 and 48 h upon laser irradiation. Conclusion: The results of this experiment demonstrated the remarkable effectiveness of NIOs/AuNPs-CS/ARI in cancer treatment, owing to their unique properties, including the PTT capability and pH sensitivity.",

keywords = "Aripiprazole, Breast cancer, Chitosan, Combination therapy, Gold nanoparticles, Niosomes, Photothermal therapy, Humans, Metal Nanoparticles/chemistry, Liposomes/chemistry, Cell Survival/drug effects, Antineoplastic Agents/chemistry, Gold/chemistry, MCF-7 Cells, Cell Line, Tumor, Female, Aripiprazole/chemistry, Chitosan/chemistry, Breast Neoplasms/drug therapy",

author = "Sajjad Alimohammadvand and Zenjanab, \{Masoumeh Kaveh\} and Pakchin, \{Parvin Samadi\} and Abdolahinia, \{Elaheh Dalir\} and Jaleh Barar and Yadollah Omidi and Pourseif, \{Mohammad M.\} and Marziyeh Fathi and Jalal Shayegh",

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year = "2024",

month = dec,

day = "24",

doi = "10.1186/s12896-024-00939-1",

language = "English",

volume = "24",

pages = "108",

journal = "BMC Biotechnology",

issn = "1472-6750",

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T1 - Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy

AU - Alimohammadvand, Sajjad

AU - Zenjanab, Masoumeh Kaveh

AU - Pakchin, Parvin Samadi

AU - Abdolahinia, Elaheh Dalir

AU - Barar, Jaleh

AU - Omidi, Yadollah

AU - Pourseif, Mohammad M.

AU - Fathi, Marziyeh

AU - Shayegh, Jalal

PY - 2024/12/24

Y1 - 2024/12/24

N2 - Introduction: Breast cancer, a formidable global health challenge for women, necessitates innovative therapeutic strategies with enhanced efficacy and minimal side effects. Aripiprazole (ARI), a widely used schizophrenia medication, exhibits promising potential in the treatment of breast cancer. As cancer therapy evolves towards a combination approach, multimodal nano-based delivery systems, such as ARI-loaded niosomes (NIOs) combined with Chitosan-Au nanoparticles for chemo-photothermal therapy, show promise over traditional chemotherapy alone by enhancing targeted efficacy and minimizing side effects. Methods: In this study, a niosomal formulation was designed, incorporating ARI and chitosan-coated AuNPs (i.e. NIOs/AuNPs-CS/ARI), to study the synergistic effect of photothermal/chemotherapy in breast cancer cells. Results: The nanosystems were characterized using UV-Vis spectroscopy and Fourier-transform infrared spectroscopy (FT-IR), confirming the successful synthesis steps. The hydrodynamic diameter of NIOs/AuNPs-CS was determined to be 44.62 nm with a zeta potential of -0.836. Also, Transmission Electron Microscopy (TEM) and Field-Emission Scanning Electron Microscopical (FE-SEM) analysis were performed to assess the size and morphology of NPs. The loading efficiency of ARI in NIOs and NIOs/AuNPs–CS was 75% and 88%, respectively. Furthermore, the release rate of the drug from NIOs/AuNPs–CS is higher than blank NIOs at two pH values (5.8 and 7.4). The cellular uptake of AuNPs-CS-encapsulated NIOs was considerably higher than that of blank NIOs. The Annexin V/PI staining assay showed that the apoptosis/necrosis rate was high in NIOs/AuNPs-CS/ARI (46%) and NIOs/ARI (36%) in 48 h. The results of MTT assessments demonstrated higher cytotoxicity by ARI-loaded NPs. The viability of MCF-7 cells treated with NIOs/AuNPs-CS/ARI was reduced from 60% and 50% to 40% and 20%, respectively, after 24 and 48 h upon laser irradiation. Conclusion: The results of this experiment demonstrated the remarkable effectiveness of NIOs/AuNPs-CS/ARI in cancer treatment, owing to their unique properties, including the PTT capability and pH sensitivity.

AB - Introduction: Breast cancer, a formidable global health challenge for women, necessitates innovative therapeutic strategies with enhanced efficacy and minimal side effects. Aripiprazole (ARI), a widely used schizophrenia medication, exhibits promising potential in the treatment of breast cancer. As cancer therapy evolves towards a combination approach, multimodal nano-based delivery systems, such as ARI-loaded niosomes (NIOs) combined with Chitosan-Au nanoparticles for chemo-photothermal therapy, show promise over traditional chemotherapy alone by enhancing targeted efficacy and minimizing side effects. Methods: In this study, a niosomal formulation was designed, incorporating ARI and chitosan-coated AuNPs (i.e. NIOs/AuNPs-CS/ARI), to study the synergistic effect of photothermal/chemotherapy in breast cancer cells. Results: The nanosystems were characterized using UV-Vis spectroscopy and Fourier-transform infrared spectroscopy (FT-IR), confirming the successful synthesis steps. The hydrodynamic diameter of NIOs/AuNPs-CS was determined to be 44.62 nm with a zeta potential of -0.836. Also, Transmission Electron Microscopy (TEM) and Field-Emission Scanning Electron Microscopical (FE-SEM) analysis were performed to assess the size and morphology of NPs. The loading efficiency of ARI in NIOs and NIOs/AuNPs–CS was 75% and 88%, respectively. Furthermore, the release rate of the drug from NIOs/AuNPs–CS is higher than blank NIOs at two pH values (5.8 and 7.4). The cellular uptake of AuNPs-CS-encapsulated NIOs was considerably higher than that of blank NIOs. The Annexin V/PI staining assay showed that the apoptosis/necrosis rate was high in NIOs/AuNPs-CS/ARI (46%) and NIOs/ARI (36%) in 48 h. The results of MTT assessments demonstrated higher cytotoxicity by ARI-loaded NPs. The viability of MCF-7 cells treated with NIOs/AuNPs-CS/ARI was reduced from 60% and 50% to 40% and 20%, respectively, after 24 and 48 h upon laser irradiation. Conclusion: The results of this experiment demonstrated the remarkable effectiveness of NIOs/AuNPs-CS/ARI in cancer treatment, owing to their unique properties, including the PTT capability and pH sensitivity.

KW - Aripiprazole

KW - Breast cancer

KW - Chitosan

KW - Combination therapy

KW - Gold nanoparticles

KW - Niosomes

KW - Photothermal therapy

KW - Humans

KW - Metal Nanoparticles/chemistry

KW - Liposomes/chemistry

KW - Cell Survival/drug effects

KW - Antineoplastic Agents/chemistry

KW - Gold/chemistry

KW - MCF-7 Cells

KW - Cell Line, Tumor

KW - Female

KW - Aripiprazole/chemistry

KW - Chitosan/chemistry

KW - Breast Neoplasms/drug therapy

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UR - https://www.scopus.com/pages/publications/85212867962#tab=citedBy

U2 - 10.1186/s12896-024-00939-1

DO - 10.1186/s12896-024-00939-1

M3 - Article

C2 - 39719556

AN - SCOPUS:85212867962

SN - 1472-6750

VL - 24

SP - 108

JO - BMC Biotechnology

JF - BMC Biotechnology

IS - 1

M1 - 108

ER -

Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

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