TY - JOUR
T1 - BAC-end Sequence Analysis and a Draft Physical Map of the Common Bean (Phaseolus vulgaris L.) Genome
AU - Schlueter, Jessica A.
AU - Goicoechea, Jose Luis
AU - Collura, Kristi
AU - Gill, Navdeep
AU - Lin, Jer-Young
AU - Yu, Yeisoo
AU - Kudrna, Dave
AU - Zuccolo, Andrea
AU - Vallejos, C. Eduardo
AU - Muñoz-Torres, Monica
AU - Blair, Matthew W.
AU - Tohme, Joe
AU - Tomkins, Jeff
AU - McClean, Phillip
AU - Wing, Rod A.
AU - Jackson, Scott A.
PY - 2008/1/15
Y1 - 2008/1/15
N2 - Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404 shotgun sequences from the cultivar Bat7 revealed that approximately 49.2% of the genome contains repetitive sequence and 29.3% is genic. Compared to other legume BAC-end sequencing projects, it appears that P. vulgaris has higher predicted levels of repetitive sequence, but this may be due to a more intense identification strategy combining both similarity-based matches as well as de novo identification of repeats. In addition, fingerprints for 41,717 BACs were obtained and assembled into a draft physical map consisting of 1,183 clone contigs and 6,385 singletons with ~9x coverage of the genome.
AB - Common bean (Phaseolus vulgaris L.) is a legume that is an important source of dietary protein in developing countries throughout the world. Utilizing the G19833 BAC library for P. vulgaris from Clemson University, 89,017 BAC-end sequences were generated giving 62,588,675 base pairs of genomic sequence covering approximately 9.54% of the genome. Analysis of these sequences in combination with 1,404 shotgun sequences from the cultivar Bat7 revealed that approximately 49.2% of the genome contains repetitive sequence and 29.3% is genic. Compared to other legume BAC-end sequencing projects, it appears that P. vulgaris has higher predicted levels of repetitive sequence, but this may be due to a more intense identification strategy combining both similarity-based matches as well as de novo identification of repeats. In addition, fingerprints for 41,717 BACs were obtained and assembled into a draft physical map consisting of 1,183 clone contigs and 6,385 singletons with ~9x coverage of the genome.
U2 - 10.1007/s12042-007-9003-9
DO - 10.1007/s12042-007-9003-9
M3 - Article
SN - 1935-9764
VL - 1
SP - 40
EP - 48
JO - Tropical Plant Biology
JF - Tropical Plant Biology
ER -