Caracterización in vitro e in vivo de tres hibridomas de interés veterinario

It were characterized the hybridomas CBSSHA.2, CBSSBm.1 and CBSSE2.1, which secrete monoclonal antibodies against the proteins hemaglutinin from Avian Influenza virus, Bm86 from Rhipicephalus (Boophilus) microplus and E2 from Classical Swine Fever virus, respectively. The class, subclass and light chain type of the antibodies were determined. The viability, cell concentration and exponential growth were evaluated in vitro. In vivo design required three groups of Balb/c mices, inoculated with 1x106, 2x106 ó 3x106 cél/mL, for study the effect of cell concentration on tumor formation, mortality and ascite production. The IgG concentrations in culture supernatants and ascites were determined by ELISA. It was evaluated the effect of inoculated cells on ascite volumes and antibody concentration. The results obtained in parameters of viability, cell concentration, exponential growth and duplication time corresponded with literature descriptions for other hybridomas. The IgG concentrations in culture supernatants and ascites were highly correlated. All hibridomas formed liquid tumors in more than 90% of inoculated mices, and the mortality was less than 10%. IgG concentration in ascite from CBSSE2.1 hybridoma depended on inoculum. In vitro and in vivo secretion of CBSSBm.1 was lesser in respect to CBSSHA.2 and CBSSE2.1.