Abstract
KleinJan et al. (Allergy 1996;51:614-20) reported that Carnoy's fixative reduced the number of chymase-positive mast cells in the nasal mucosa. Therefore, in the present study, we investigated whether Carnoy's fixative reduces the number of chymase-positive cells from cord-blood-derived human cultured mast cells when compared with other types of fixatives. Human mast cells were obtained by culturing cord-blood-derived CD34 positive cells in the presence: of stem cell factor and interleukin-6. Staining procedures of the cells in fixation with Carnoy's fixative and with other fixatives gave no differences among the number of tryptase-positive cells, whereas fixation with Carnoy's fixative for 15 min gave a significant decrease in the number of chymase-positive cells compared with acetone for 10 min. The number of chymase-positive cells decreased in a time-dependent manner under fixation with Carnoy's fixative, indicating that Carnoy's fixative had a negative effect on the number of chymase-positive cells from cord-blood derived human cultured mast cells.
| Original language | English |
|---|---|
| Pages (from-to) | 981-985 |
| Number of pages | 5 |
| Journal | Allergy: European Journal of Allergy and Clinical Immunology |
| Volume | 53 |
| Issue number | 10 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
ASJC Scopus Subject Areas
- Immunology and Allergy
- Immunology
Keywords
- Acetone
- Carnoy's fixative
- Chymase
- Immunocytochemical staining
- Mast cell
- Tryptase
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