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Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling

  • Richard H. Perry
  • , David I. Bellovin
  • , Emelyn H. Shroff
  • , Ali I. Ismail
  • , Tahera Zubuawala
  • , Dean W. Felsher
  • , Richard N. Zare

Research output: Contribution to journalArticlepeer-review

Abstract

We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis. © 2013 American Chemical Society.
Original languageAmerican English
Pages (from-to)4259-4262
Number of pages4
JournalAnalytical Chemistry
Volume85
Issue number9
DOIs
StatePublished - May 7 2013
Externally publishedYes

Funding

FundersFunder number
National Cancer InstituteR01CA089305

    ASJC Scopus Subject Areas

    • Analytical Chemistry

    Keywords

    • Animals
    • Spectrometry, Mass, Electrospray Ionization
    • Carcinoma, Hepatocellular/genetics
    • Mice, Transgenic
    • Proto-Oncogene Proteins c-myc/genetics
    • Lipid Metabolism/genetics
    • Mice
    • Lipids/genetics
    • Liver Neoplasms/genetics
    • Carcinogenesis/genetics
    • Disease Models, Animal

    Disciplines

    • Chemistry
    • Medicine and Health Sciences
    • Physical Sciences and Mathematics

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