Commensal Streptococcus mitis is a unique vector for oral mucosal vaccination

Nada Daifalla; Mark J. Cayabyab; Emily Xie; Hyeun Bum Kim; Saul Tzipori; Philip Stashenko; Margaret Duncan; Antonio Campos-Neto

doi:10.1016/j.micinf.2014.11.002

Commensal Streptococcus mitis is a unique vector for oral mucosal vaccination

Nada Daifalla
, Mark J. Cayabyab
, Emily Xie
, Hyeun Bum Kim
, Saul Tzipori
, Philip Stashenko
, Margaret Duncan
, Antonio Campos-Neto

Research output: Contribution to journal › Article › peer-review

Abstract

The development of vaccine approaches that induce mucosal and systemic immune responses is critical for the effective prevention of several infections. Here, we report on the use of the abundant human oral commensal bacterium Streptococcus mitis as a delivery vehicle for mucosal immunization. Using homologous recombination we generated a stable rS. mitis expressing a Mycobacterium tuberculosis protein (Ag85b). Oral administration of rS. mitis in gnotobiotic piglets resulted in efficient oral colonization and production of oral and systemic anti-Ag85b specific IgA and IgG antibodies. These results support that the commensal S. mitis is potentially a useful vector for mucosal vaccination.

Original language	English
Pages (from-to)	237-242
Number of pages	6
Journal	Microbes and Infection
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/j.micinf.2014.11.002
State	Published - Mar 1 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 Institut Pasteur.

ASJC Scopus Subject Areas

Microbiology
Immunology
Infectious Diseases

Keywords

Ag85b
Gnotobiotic piglets
Mucosal vaccine
Oral immunization
Streptococcus mitis
Vaccine vector

Access to Document

10.1016/j.micinf.2014.11.002

Cite this

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title = "Commensal Streptococcus mitis is a unique vector for oral mucosal vaccination",

abstract = "The development of vaccine approaches that induce mucosal and systemic immune responses is critical for the effective prevention of several infections. Here, we report on the use of the abundant human oral commensal bacterium Streptococcus mitis as a delivery vehicle for mucosal immunization. Using homologous recombination we generated a stable rS. mitis expressing a Mycobacterium tuberculosis protein (Ag85b). Oral administration of rS. mitis in gnotobiotic piglets resulted in efficient oral colonization and production of oral and systemic anti-Ag85b specific IgA and IgG antibodies. These results support that the commensal S. mitis is potentially a useful vector for mucosal vaccination.",

keywords = "Ag85b, Gnotobiotic piglets, Mucosal vaccine, Oral immunization, Streptococcus mitis, Vaccine vector",

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doi = "10.1016/j.micinf.2014.11.002",

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AU - Daifalla, Nada

AU - Cayabyab, Mark J.

AU - Xie, Emily

AU - Kim, Hyeun Bum

AU - Tzipori, Saul

AU - Stashenko, Philip

AU - Duncan, Margaret

AU - Campos-Neto, Antonio

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AB - The development of vaccine approaches that induce mucosal and systemic immune responses is critical for the effective prevention of several infections. Here, we report on the use of the abundant human oral commensal bacterium Streptococcus mitis as a delivery vehicle for mucosal immunization. Using homologous recombination we generated a stable rS. mitis expressing a Mycobacterium tuberculosis protein (Ag85b). Oral administration of rS. mitis in gnotobiotic piglets resulted in efficient oral colonization and production of oral and systemic anti-Ag85b specific IgA and IgG antibodies. These results support that the commensal S. mitis is potentially a useful vector for mucosal vaccination.

KW - Ag85b

KW - Gnotobiotic piglets

KW - Mucosal vaccine

KW - Oral immunization

KW - Streptococcus mitis

KW - Vaccine vector

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Commensal Streptococcus mitis is a unique vector for oral mucosal vaccination

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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