Complete Genome Sequences of Genamy16 and NovaSharks, Two Gordonia rubripertincta Bacteriophages Isolated from Soil in Southeastern Florida

Julie Torruellas Garcia; Sarah Ballarin; Neel Balusa; Melissa Bell; Samia Caballero; Joshua Chan; Maria Farez; Ashley J. Guillen-Tapia; Kristin Parent; Nashrah Pierre-Louis; Victoria Polishuk; Bhavya Soni; Sundharraman Subramanian; Katie E. Crump

doi:10.1128/mra.00973-22

Complete Genome Sequences of Genamy16 and NovaSharks, Two Gordonia rubripertincta Bacteriophages Isolated from Soil in Southeastern Florida

Julie Torruellas Garcia
, Sarah Ballarin
, Neel Balusa
, Melissa Bell
, Samia Caballero
, Joshua Chan
, Maria Farez
, Ashley J. Guillen-Tapia
, Kristin Parent
, Nashrah Pierre-Louis
, Victoria Polishuk
, Bhavya Soni
, Sundharraman Subramanian
, Katie E. Crump

Department of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

We report on two actinobacteriophages, Genamy16 and NovaSharks, that were isolated from soil in Florida using Gordonia rubripertincta NRRL B-16540. The genomes of both phages are ~65,000 bp, with similar GC contents, and, based on gene content similarity to phages in the Actinobacteriophage Database, were assigned to phage cluster DV. We report on two actinobacteriophages, Genamy16 and NovaSharks, that were isolated from soil in Florida using Gordonia rubripertincta NRRL B-16540. The genomes of both phages are ~65,000 bp, with similar GC contents, and, based on gene content similarity to phages in the Actinobacteriophage Database, were assigned to phage cluster DV.

Original language	American English
Number of pages	4
Journal	Microbiology Resource Announcements
Volume	11
Issue number	12
DOIs	https://doi.org/10.1128/mra.00973-22 https://doi.org/10.1128/mra.00973-22
State	Published - Dec 15 2022

Bibliographical note

Publisher Copyright:
Copyright © 2022 Torruellas Garcia et al.

Funding

This study was supported by the Department of Biological Sciences, Halmos College of Arts and Sciences at Nova Southeastern University, the Howard Hughes Medical Institute Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program, and the University of Pittsburgh. S.S. was supported by National Science Foundation Career Award 1750125. This study was supported by the Department of Biological Sciences, Halmos College of Arts and Sciences at Nova Southeastern University, the Howard Hughes Medical Institute Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program, and the University of Pittsburgh. S.S. was supported by National Science Foundation Career Award 1750125. We thank Viknesh Sivanathan, senior program lead at the Howard Hughes Medical Institute, for his assistance in extracting the phage DNA. We also thank the Michigan State University Research Technology Support Facility (RTSF) Cryo-EM Core Facility for the use of the Talos Arctica system.

Funders	Funder number
Department of Biological Sciences
Halmos College of Arts and Sciences at Nova Southeastern University
Howard Hughes Medical Institute Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
Michigan State University Research Technology Support Facility
RTSF
SEA-PHAGES
National Science Foundation	1750125
University of Pittsburgh

ASJC Scopus Subject Areas

Immunology and Microbiology (miscellaneous)
Genetics
Molecular Biology

Disciplines

Biology
Life Sciences

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https://journals.asm.org/doi/pdf/10.1128/mra.00973-22

Cite this

Torruellas Garcia, J., Ballarin, S., Balusa, N., Bell, M., Caballero, S., Chan, J., Farez, M., Guillen-Tapia, A. J., Parent, K., Pierre-Louis, N., Polishuk, V., Soni, B., Subramanian, S., & Crump, K. E. (2022). Complete Genome Sequences of Genamy16 and NovaSharks, Two Gordonia rubripertincta Bacteriophages Isolated from Soil in Southeastern Florida. Microbiology Resource Announcements, 11(12). https://doi.org/10.1128/mra.00973-22, https://doi.org/10.1128/mra.00973-22

Torruellas Garcia, J, Ballarin, S, Balusa, N, Bell, M, Caballero, S, Chan, J, Farez, M, Guillen-Tapia, AJ, Parent, K, Pierre-Louis, N, Polishuk, V, Soni, B, Subramanian, S & Crump, KE 2022, 'Complete Genome Sequences of Genamy16 and NovaSharks, Two Gordonia rubripertincta Bacteriophages Isolated from Soil in Southeastern Florida', Microbiology Resource Announcements, vol. 11, no. 12. https://doi.org/10.1128/mra.00973-22, https://doi.org/10.1128/mra.00973-22

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title = "Complete Genome Sequences of Genamy16 and NovaSharks, Two Gordonia rubripertincta Bacteriophages Isolated from Soil in Southeastern Florida",

abstract = " We report on two actinobacteriophages, Genamy16 and NovaSharks, that were isolated from soil in Florida using Gordonia rubripertincta NRRL B-16540. The genomes of both phages are \textasciitilde{}65,000 bp, with similar GC contents, and, based on gene content similarity to phages in the Actinobacteriophage Database, were assigned to phage cluster DV. We report on two actinobacteriophages, Genamy16 and NovaSharks, that were isolated from soil in Florida using Gordonia rubripertincta NRRL B-16540. The genomes of both phages are \textasciitilde{}65,000 bp, with similar GC contents, and, based on gene content similarity to phages in the Actinobacteriophage Database, were assigned to phage cluster DV. ",

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Complete Genome Sequences of Genamy16 and NovaSharks, Two Gordonia rubripertincta Bacteriophages Isolated from Soil in Southeastern Florida

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

Disciplines

Access to Document

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