Development and Characterization of 11 Novel Microsatellite Loci for the Roundscale Spearfish Tetrapturus georgii and Their Cross-Species Amplification Among Other Istiophorid Species

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Abstract

Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity (HE) of 0·09-0·94, and all loci conformed to Hardy-Weinberg expectations. Cross-amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.
Original languageAmerican English
Pages (from-to)1781-1786
Number of pages6
JournalJournal of Fish Biology
Volume81
DOIs
StatePublished - Sep 14 2012

ASJC Scopus Subject Areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Keywords

  • Atlantic
  • Billfish
  • Istiophoridae
  • Population genetics
  • Primer sequence

Disciplines

  • Genetics and Genomics
  • Marine Biology
  • Oceanography and Atmospheric Sciences and Meteorology

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