Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species

Andrea M. Bernard; Kevin A. Feldheim; Vincent P. Richards; Richard S. Nemeth; Mahmood S. Shivji

doi:10.1007/s12686-012-9688-4

Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species

Andrea M. Bernard
, Kevin A. Feldheim
, Vincent P. Richards
, Richard S. Nemeth
, Mahmood S. Shivji

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The Nassau grouper (Epinephelus striatus), a severely overexploited and endangered coral reef fish in the Western Atlantic, is in urgent need of conservation and management measures. We report the development and characterization of 15 polymorphic, mostly tetranucleotide microsatellite loci based on 40 Nassau grouper samples collected from two Caribbean spawning aggregations. The number of alleles per locus and expected heterozygosities ranged from 4 to 19 and 0. 40 to 0. 92, respectively. We also demonstrate the cross-amplification utility of these microsatellites for genetic studies of five other grouper species of conservation interest. © 2012 Springer Science+Business Media B.V.

Original language	American English
Pages (from-to)	983-986
Number of pages	4
Journal	Conservation Genetics Resources
Volume	4
DOIs	https://doi.org/10.1007/s12686-012-9688-4
State	Published - Jun 17 2012

Funding

Acknowledgments This project was funded by the US Geological Survey (Project #07ERAG0078), NOAA Center for Sponsored Coastal Ocean Science award #NA10NOS4260221 to the National Coral Reef Institute, NOAA Pulley Ridge Connectivity Project, the Guy Harvey Ocean Foundation, and a National Science and Engineering Council of Canada Graduate Fellowship (AMB). We thank E. Kadison, J. Blondeau, B. Semmens, A. Jackson and L. Bullock for grouper samples. This is contribution 87 from the CMES-UVI.

Funders	Funder number
National Oceanic and Atmospheric Administration	10NOS4260221
U.S. Geological Survey	07ERAG0078
Guy Harvey Ocean Foundation
Natural Sciences and Engineering Research Council of Canada

ASJC Scopus Subject Areas

Genetics
Ecology, Evolution, Behavior and Systematics

Keywords

Grouper genetics
Grouper conservation
Serranidae
Epinephelus
Mycteroperca

Disciplines

Genetics and Genomics
Marine Biology
Oceanography and Atmospheric Sciences and Meteorology

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10.1007/s12686-012-9688-4

Cite this

Bernard, A. M., Feldheim, K. A., Richards, V. P., Nemeth, R. S., & Shivji, M. S. (2012). Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species. Conservation Genetics Resources, 4, 983-986. https://doi.org/10.1007/s12686-012-9688-4

Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species. / Bernard, Andrea M.; Feldheim, Kevin A.; Richards, Vincent P. et al.
In: Conservation Genetics Resources, Vol. 4, 17.06.2012, p. 983-986.

Research output: Contribution to journal › Article › peer-review

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Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species

Abstract

Funding

ASJC Scopus Subject Areas

Keywords

Disciplines

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