Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)

Andrea M. Bernard; A. W. S. Hilsdorf; A. F. Amorim; Rodrigo Rodrigues Domingues; Mahmood S. Shivji

doi:10.1007/s12686-013-0038-y

Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)

Andrea M. Bernard
, A. W. S. Hilsdorf
, A. F. Amorim
, Rodrigo Rodrigues Domingues
, Mahmood S. Shivji

Research output: Contribution to journal › Article › peer-review

Abstract

The longbill spearfish ( Tetrapturus pfluegeri ; Istiophoridae) is an epipelagic billfish species broadly distributed in the Atlantic Ocean and exploited by multinational fisheries. No data exist on the population structure or genetic diversity of this apex predator, despite a strong need for this information to inform international conservation and management efforts. Thirteen microsatellite loci, comprising mostly tri- and tetra-nucleotide repeats, were isolated and characterized for this species by genotyping individuals (n = 29–42) obtained from western North and South Atlantic fisheries. The average number of alleles ranged from 3 to 16 and the expected heterozygosity ranged from 0.35 to 0.86. This suite of markers provides the first population genetic nuclear resources for the longbill spearfish, allowing initiation of studies for assessing the population structure and demography of this poorly understood species.

Original language	American English
Pages (from-to)	169-171
Number of pages	3
Journal	Conservation Genetics Resources
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/s12686-013-0038-y
State	Published - Sep 28 2013

Bibliographical note

Publisher Copyright:
© 2013, Springer Science+Business Media Dordrecht.

Funding

Acknowledgments We thank L. Beerkircher of the National Marine Fisheries Service for providing longbill spearfish tissue samples. This research was funded by an NSERC Fellowship to AMB, a grant from FAPESP (2009/54.660-6), and operational funds to the Guy Harvey Research Institute from the Guy Harvey Ocean Foundation.

Funders
Natural Sciences and Engineering Research Council of Canada
Fundação de Amparo à Pesquisa do Estado de São Paulo

ASJC Scopus Subject Areas

Genetics
Ecology, Evolution, Behavior and Systematics

Keywords

Billfish genetics
Billfish conservation and management
Istiophoridae
STR

Disciplines

Genetics and Genomics
Marine Biology
Oceanography and Atmospheric Sciences and Meteorology

Access to Document

10.1007/s12686-013-0038-y

Cite this

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title = "Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)",

abstract = " The longbill spearfish ( Tetrapturus pfluegeri ; Istiophoridae) is an epipelagic billfish species broadly distributed in the Atlantic Ocean and exploited by multinational fisheries. No data exist on the population structure or genetic diversity of this apex predator, despite a strong need for this information to inform international conservation and management efforts. Thirteen microsatellite loci, comprising mostly tri- and tetra-nucleotide repeats, were isolated and characterized for this species by genotyping individuals (n = 29–42) obtained from western North and South Atlantic fisheries. The average number of alleles ranged from 3 to 16 and the expected heterozygosity ranged from 0.35 to 0.86. This suite of markers provides the first population genetic nuclear resources for the longbill spearfish, allowing initiation of studies for assessing the population structure and demography of this poorly understood species.",

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doi = "10.1007/s12686-013-0038-y",

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AU - Hilsdorf, A. W. S.

AU - Amorim, A. F.

AU - Rodrigues Domingues, Rodrigo

AU - Shivji, Mahmood S.

PY - 2013/9/28

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KW - Billfish conservation and management

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Development and Characterization of Thirteen Microsatellite Markers for the Longbill Spearfish (Tetrapturus pfluegeri)

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

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