Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo-produced Beauveria bassiana cells

Aurelien Tartar; A. M. Shapiro; D. W. Scharf; Drion G. Boucias

doi:10.1007/s11046-005-0134-y

Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo-produced Beauveria bassiana cells

Aurelien Tartar
, A. M. Shapiro
, D. W. Scharf
, Drion G. Boucias

Research output: Contribution to journal › Article › peer-review

Abstract

During infection (in vivo), the entomopathogenic fungus Beauveria bassiana produces yeast-like cells that are surrounded by modified cell walls. These modifications have been related to the fungus ability to limit recognition by the host defense system. The composition of the in vivo cell wall was analyzed using a combination of cytochemical and molecular techniques. The in vivo cell walls still contained both chitin and 1,3-β-glucan, but they were significantly thinner than in vitro cell walls (50-60 nm versus 100-160 nm, respectively). The difference in cell wall thickness was correlated with transcriptional regulation of cell wall-related genes: quantitative RT-PCR reactions demonstrated that B. bassiana chitin synthase (CHS) and glucan synthase (FKS) genes are down regulated in vivo. These analyses indicate that in vivo-triggered phenotypic modifications, including cell wall adjustments, are controlled by molecular mechanisms that include regulation of gene expression at the transcriptional level.

Original language	American English
Pages (from-to)	303-314
Number of pages	12
Journal	Mycopathologia
Volume	160
Issue number	4
DOIs	https://doi.org/10.1007/s11046-005-0134-y
State	Published - Jan 1 2005

Funding

The authors thank David Hibbett (Clark University) and Nemat Keyhani (University of Florida) for critical comments on the manuscript; the ICBR Sequencing Facility at the University of Florida; and Michael Scharf for providing access to the iCycler iQ real-time PCR detection system. This work is supported by a grant from the National Science Foundation (NSF EF-0412137). Florida Agricultural Experiment Station Journal Series No. R-10754

Funders	Funder number
National Science Foundation	NSF EF-0412137

ASJC Scopus Subject Areas

Applied Microbiology and Biotechnology
Agronomy and Crop Science
veterinary (miscalleneous)
Microbiology

Keywords

AY743592
AY743593
Fungal cell wall
CF350282
Dimorphism
Host-pathogen interactions

Disciplines

Biology

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10.1007/s11046-005-0134-y

Cite this

Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo-produced Beauveria bassiana cells. / Tartar, Aurelien; Shapiro, A. M.; Scharf, D. W. et al.
In: Mycopathologia, Vol. 160, No. 4, 01.01.2005, p. 303-314.

Research output: Contribution to journal › Article › peer-review

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title = "Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo-produced Beauveria bassiana cells",

abstract = "During infection (in vivo), the entomopathogenic fungus Beauveria bassiana produces yeast-like cells that are surrounded by modified cell walls. These modifications have been related to the fungus ability to limit recognition by the host defense system. The composition of the in vivo cell wall was analyzed using a combination of cytochemical and molecular techniques. The in vivo cell walls still contained both chitin and 1,3-β-glucan, but they were significantly thinner than in vitro cell walls (50-60 nm versus 100-160 nm, respectively). The difference in cell wall thickness was correlated with transcriptional regulation of cell wall-related genes: quantitative RT-PCR reactions demonstrated that B. bassiana chitin synthase (CHS) and glucan synthase (FKS) genes are down regulated in vivo. These analyses indicate that in vivo-triggered phenotypic modifications, including cell wall adjustments, are controlled by molecular mechanisms that include regulation of gene expression at the transcriptional level.",

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AU - Scharf, D. W.

AU - Boucias, Drion G.

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AB - During infection (in vivo), the entomopathogenic fungus Beauveria bassiana produces yeast-like cells that are surrounded by modified cell walls. These modifications have been related to the fungus ability to limit recognition by the host defense system. The composition of the in vivo cell wall was analyzed using a combination of cytochemical and molecular techniques. The in vivo cell walls still contained both chitin and 1,3-β-glucan, but they were significantly thinner than in vitro cell walls (50-60 nm versus 100-160 nm, respectively). The difference in cell wall thickness was correlated with transcriptional regulation of cell wall-related genes: quantitative RT-PCR reactions demonstrated that B. bassiana chitin synthase (CHS) and glucan synthase (FKS) genes are down regulated in vivo. These analyses indicate that in vivo-triggered phenotypic modifications, including cell wall adjustments, are controlled by molecular mechanisms that include regulation of gene expression at the transcriptional level.

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Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo-produced Beauveria bassiana cells

Abstract

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Keywords

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