Evaluación in vitro de la microfiltración de Enterococcus faecalis usando cinco técnicas de obturación

ABSTRACT. Background: The root canal obturation technique known as “hybrid-mixed condensation” combines the advantages of the lateral (cold) technique with the thermoplastic (heat) technique. To date, its sealing effectiveness has not been studied. Purpose: To evaluate the sealing capacity of root canals obturated with the following techniques: lateral condensation, vertical condensation, single cone, Guttacore® carrier, and hybrid-mixed condensation. Methods: Fifty premolars extracted for orthodontic reasons and prepared with a WaveOne® Primary file were divided into five groups and obturated with: a non-standardized WaveOne® Primary single cone, lateral condensation using 2% gutta-percha cones, size 30 Guttacore® obturators, vertical condensation with a WaveOne® Primary cone, and injection of alpha-type gutta-percha from the Beefill® system. Hybrid-mixed condensation was performed, modifying lateral condensation with heat and injecting Beefill® alpha gutta-percha. Enterococcus faecalis was inoculated through the coronal portion. Microleakage was determined by color change and turbidity in the phenol red culture broth. Specimens were incubated at 37 ° C for 12 weeks. They were then examined by scanning electron microscopy. Results: 11 specimens (22%) exhibited leakage. Leakage occurred in 46% of specimens with a single cone, 30% with Gutacore®, 20% with lateral condensation, 10% with the vertical condensation technique, and none with hybrid-mixed condensation. Conclusion: The hybrid-mixed condensation technique proved to be the most efficient for achieving adequate sealing and preventing microbial contamination of the canals during endodontic treatment.