Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium

Taylor Morrisette; Katherine L. Lev; Gregory S. Canfield; Breck A. Duerkop; Razieh Kebriaei; Kyle C. Stamper; Dana Holger; Susan M. Lehman; Smaranda Willcox; Cesar A. Arias; Michael J. Rybak

doi:10.1128/AAC.01623-21

Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium

Taylor Morrisette
, Katherine L. Lev
, Gregory S. Canfield
, Breck A. Duerkop
, Razieh Kebriaei
, Kyle C. Stamper
, Dana Holger
, Susan M. Lehman
, Smaranda Willcox
, Cesar A. Arias
, Michael J. Rybak

Research output: Contribution to journal › Article › peer-review

Abstract

Enterococcus faecium is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. faecium strains against 4 phages, 2 phages (113 and 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, wholegenome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin (DAP) plus the phage cocktail (113 [myophage] and 9184 [siphopage]) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin-nonsusceptible E. faecium.

Original language	English
Article number	e01623-21
Journal	Antimicrobial Agents and Chemotherapy
Volume	66
Issue number	1
DOIs	https://doi.org/10.1128/AAC.01623-21
State	Published - Jan 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022 American Society for Microbiology.

ASJC Scopus Subject Areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Keywords

Bacteriophage cocktails
Bacteriophage-antibiotic combinations
Bacteriophages
Daptomycin
Enterococcus faecium

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10.1128/AAC.01623-21

Cite this

Morrisette, T., Lev, K. L., Canfield, G. S., Duerkop, B. A., Kebriaei, R., Stamper, K. C., Holger, D., Lehman, S. M., Willcox, S., Arias, C. A., & Rybak, M. J. (2022). Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium. Antimicrobial Agents and Chemotherapy, 66(1), Article e01623-21. https://doi.org/10.1128/AAC.01623-21

Morrisette, T, Lev, KL, Canfield, GS, Duerkop, BA, Kebriaei, R, Stamper, KC, Holger, D, Lehman, SM, Willcox, S, Arias, CA & Rybak, MJ 2022, 'Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium', Antimicrobial Agents and Chemotherapy, vol. 66, no. 1, e01623-21. https://doi.org/10.1128/AAC.01623-21

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title = "Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium",

abstract = "Enterococcus faecium is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. faecium strains against 4 phages, 2 phages (113 and 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, wholegenome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin (DAP) plus the phage cocktail (113 [myophage] and 9184 [siphopage]) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin-nonsusceptible E. faecium.",

keywords = "Bacteriophage cocktails, Bacteriophage-antibiotic combinations, Bacteriophages, Daptomycin, Enterococcus faecium",

author = "Taylor Morrisette and Lev, \{Katherine L.\} and Canfield, \{Gregory S.\} and Duerkop, \{Breck A.\} and Razieh Kebriaei and Stamper, \{Kyle C.\} and Dana Holger and Lehman, \{Susan M.\} and Smaranda Willcox and Arias, \{Cesar A.\} and Rybak, \{Michael J.\}",

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month = jan,

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language = "English",

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journal = "Antimicrobial Agents and Chemotherapy",

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AU - Duerkop, Breck A.

AU - Kebriaei, Razieh

AU - Stamper, Kyle C.

AU - Holger, Dana

AU - Lehman, Susan M.

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KW - Bacteriophage-antibiotic combinations

KW - Bacteriophages

KW - Daptomycin

KW - Enterococcus faecium

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Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin against Daptomycin-Nonsusceptible Enterococcus faecium

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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