Abstract
Objective: The variability in reports associating Enterococcus faecalis with endodontic treatment failure is due to its remarkable genetic plasticity and clonal diversity facilitating the adaptation to adverse microenvironments. Materials and Methods: Present study approximated the genetic diversity of Enterococcus faecalis isolates from oral cavity samples of endodontic origin; the detection involves genes Asa, Esp, EfaA, Ace, Asa373, GelE, and CylA, and mature biofilm formation. Results: On the other side, the genetic clusters were analysed based on the sequence alignment of the amplified product of a 16S rDNA PCR. Results showed that the genetic clustering of Enterococcus faecalis isolates was distinct between patients but with a high degree of similarity between samples from the same individual. Conclusion: The virulence genes presence and biofilm formation resulted independent of genetic clustering but related to sample origin.
| Original language | Undefined/Unknown |
|---|---|
| Article number | 131 |
| Number of pages | 8 |
| Journal | Japan Journal of Research |
| Volume | 6 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2025 |
Funding
This research was sponsored by the “Vicerrectoría de Investigación de la Pontificia Universidad Javeriana”, ID: 00007914 - SIAP 00007699. The study had the institutional endorsement of the Ethics Committee of the Faculty of Sciences and the Faculty of Dentistry. All included patients signed an informed consent.
Keywords
- Enterococcus faecalis
- biofilm
- phylogeny
- genetic clustering
- virulence genes
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