Exploring the diagnostic potential of immune biomarker coexpression in Gulf War Illness.

G Broderick; Mary A Fletcher; M Gallagher; Z M Barnes; S D Vernon; Nancy G. Klimas

doi:10.1007/978-1-62703-071-7_8

Exploring the diagnostic potential of immune biomarker coexpression in Gulf War Illness.

G Broderick
, Mary A Fletcher
, M Gallagher
, Z M Barnes
, S D Vernon
, Nancy G. Klimas

College of Psychology

Research output: Contribution to journal › Article › peer-review

Abstract

Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%. © 2012 Springer Science+Business Media, LLC.

Original language	American English
Pages (from-to)	145-164
Number of pages	20
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	934
DOIs	https://doi.org/10.1007/978-1-62703-071-7_8
State	Published - Jan 1 2012

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Medicine and Health Sciences
Osteopathic Medicine and Osteopathy

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Cite this

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abstract = "Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70\% and a specificity of 90\%. {\textcopyright} 2012 Springer Science+Business Media, LLC.",

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AU - Broderick, G

AU - Fletcher, Mary A

AU - Gallagher, M

AU - Barnes, Z M

AU - Vernon, S D

AU - Klimas, Nancy G.

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AB - Complex disorders like Gulf War Illness (GWI) often defy diagnosis on the basis of a single biomarker and may only be distinguishable by considering the coexpression of multiple markers measured in response to a challenge. We demonstrate the practical application of such an approach using an example where blood was collected from 26 GWI, 13 healthy control subjects, and 9 unhealthy controls with Chronic Fatigue at three points during a graded exercise challenge. A 3-way multivariate projection model based on 12 markers of endocrine and immune function was constructed using a training set of n = 10 GWI and n = 11 healthy controls. These groups were separated almost completely on the basis of two coexpression patterns. In a separate test set these same features allowed for discrimination of new GWI subjects (n = 16) from unhealthy (n = 9) and healthy control subjects with a sensitivity of 70% and a specificity of 90%. © 2012 Springer Science+Business Media, LLC.

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Exploring the diagnostic potential of immune biomarker coexpression in Gulf War Illness.

Abstract

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