Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

George T. Duncan; Kuppareddi Balamurugan; Bruce Budowle; Martin L. Tracey

doi:10.1016/S1050-3862(96)00159-3

Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

George T. Duncan
, Kuppareddi Balamurugan
, Bruce Budowle
, Martin L. Tracey

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.

Original language	English
Pages (from-to)	119-121
Number of pages	3
Journal	Genetic Analysis - Biomolecular Engineering
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/S1050-3862(96)00159-3
State	Published - Nov 1996
Externally published	Yes

ASJC Scopus Subject Areas

Applied Microbiology and Biotechnology
Genetics

Keywords

D1S80
Forensics
Minisatellite
Polymorphism
Sequence
Variable number tandem repeat (VNTR)

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10.1016/S1050-3862(96)00159-3

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title = "Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80",

abstract = "The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.",

keywords = "D1S80, Forensics, Minisatellite, Polymorphism, Sequence, Variable number tandem repeat (VNTR)",

author = "Duncan, \{George T.\} and Kuppareddi Balamurugan and Bruce Budowle and Tracey, \{Martin L.\}",

year = "1996",

month = nov,

doi = "10.1016/S1050-3862(96)00159-3",

language = "English",

volume = "13",

pages = "119--121",

journal = "Genetic Analysis - Biomolecular Engineering",

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publisher = "Elsevier B.V.",

number = "5",

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TY - JOUR

T1 - Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

AU - Duncan, George T.

AU - Balamurugan, Kuppareddi

AU - Budowle, Bruce

AU - Tracey, Martin L.

PY - 1996/11

Y1 - 1996/11

N2 - The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.

AB - The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.

KW - D1S80

KW - Forensics

KW - Minisatellite

KW - Polymorphism

KW - Sequence

KW - Variable number tandem repeat (VNTR)

UR - https://www.scopus.com/pages/publications/16344384893

UR - https://www.scopus.com/pages/publications/16344384893#tab=citedBy

U2 - 10.1016/S1050-3862(96)00159-3

DO - 10.1016/S1050-3862(96)00159-3

M3 - Article

C2 - 9021400

AN - SCOPUS:16344384893

SN - 1050-3862

VL - 13

SP - 119

EP - 121

JO - Genetic Analysis - Biomolecular Engineering

JF - Genetic Analysis - Biomolecular Engineering

IS - 5

ER -

Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

Abstract

ASJC Scopus Subject Areas

Keywords

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