Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate

Mikihito Kajiya; Marcelo J.B. Silva; Kimihiro Sato; Kazuhisa Ouhara; Toshihisa Kawai

doi:10.1016/j.bbrc.2009.05.117

Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate

Mikihito Kajiya
, Marcelo J.B. Silva
, Kimihiro Sato
, Kazuhisa Ouhara
, Toshihisa Kawai

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

By its antioxidant effect, molecular hydrogen gas (H₂) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H₂, or (3) H₂ only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-α and IL-1β in colon lesion, were significantly suppressed by the addition of H₂ to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H₂. Therefore, the present study indicated that H₂ can prevent the development of DSS-induced colitis in mice.

Original language	English
Pages (from-to)	11-15
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	386
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.05.117
State	Published - Aug 14 2009
Externally published	Yes

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Keywords

Antioxidant
Colitis
Colon
Dextran sodium sulfate
IL-1-beta
IL-12
Inflammation
Macrophages
Molecular hydrogen
TNF-alpha

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10.1016/j.bbrc.2009.05.117

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title = "Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate",

abstract = "By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5\%), (2) DSS (5\%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-α and IL-1β in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.",

keywords = "Antioxidant, Colitis, Colon, Dextran sodium sulfate, IL-1-beta, IL-12, Inflammation, Macrophages, Molecular hydrogen, TNF-alpha",

author = "Mikihito Kajiya and Silva, \{Marcelo J.B.\} and Kimihiro Sato and Kazuhisa Ouhara and Toshihisa Kawai",

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doi = "10.1016/j.bbrc.2009.05.117",

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AU - Kajiya, Mikihito

AU - Silva, Marcelo J.B.

AU - Sato, Kimihiro

AU - Ouhara, Kazuhisa

AU - Kawai, Toshihisa

PY - 2009/8/14

Y1 - 2009/8/14

N2 - By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-α and IL-1β in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.

AB - By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-α and IL-1β in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.

KW - Antioxidant

KW - Colitis

KW - Colon

KW - Dextran sodium sulfate

KW - IL-1-beta

KW - IL-12

KW - Inflammation

KW - Macrophages

KW - Molecular hydrogen

KW - TNF-alpha

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C2 - 19486890

AN - SCOPUS:67649198933

SN - 0006-291X

VL - 386

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

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Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate

Abstract

ASJC Scopus Subject Areas

Keywords

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