Improved immobilization of gelatin on a modified polyurethane urea

In this study, polyurethane urea was surface-modified to elevate cell recognition through immobilization of bioactive gelatin. The poly(urethane urea) was synthesized using poly(ε-caprolactone) diol in the absence of a chain extender. The synthesized polyurethane urea was then functionalized with gelatin (gelatin-grafted poly(urethane urea)) via aminolysis. Chemical changes at the polyurethane urea surface were monitored using titration, water contact angle. Fourier transform infrared, and zeta potential measurements. Significantly larger amounts of gelatin were grafted on the polyurethane urea surface compared to those previously reported for poly(ε-caprolactone) diol (three times more) and polyurethanes (two times more), while the mechanical properties were not compromised. Proliferation of human adipose–derived mesenchymal stem cells on the polyurethane urea and the gelatin-grafted polyurethane urea was evaluated through MTT assay. Although both samples enhanced human adipose–derived mesenchymal stem cells’ proliferation, gelatin-grafted polyurethane urea supported human adipose–derived mesenchymal stem cells’ proliferation at a remarkably higher rate.