Influence of chirality of V(V) Schiff base complexes on DNA, BSA binding and cleavage activity.

N U Khan; Naushira Pandya; Maity NCh; M Kumar; R M Patel; R I Kureshy; S H Abdi; S Mishra; S Das; H C Bajaj

doi:10.1016/j.ejmech.2011.08.020

Influence of chirality of V(V) Schiff base complexes on DNA, BSA binding and cleavage activity.

N U Khan
, Naushira Pandya
, Maity NCh
, M Kumar
, R M Patel
, R I Kureshy
, S H Abdi
, S Mishra
, S Das
, H C Bajaj

Research output: Contribution to journal › Article › peer-review

Abstract

New chiral V(V) Schiff base complexes (S)-[VO(OMe)L] and (R)-[VO(OMe)L] were synthesized and characterized by microanalysis, infrared (IR), UV-Visible, Circular dichroism (CD) spectroscopy and single crystal X-ray studies. The interaction of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA) protein showed chiral expression DNA/protein binding strength. The influence of chirality was also observed in cytotoxicity assay of Hep 2 cells. (R)-[VO(OMe)L] enantiomer exhibited higher binding constant (5 ± 1 × 10 5 M -1) as compared to (S)-[VO(OMe)L] (8 ± 1 × 10 4 M -1). The fluorescence quenching, thermal melting and viscosity data suggest DNA surface and/or groove binding nature of the complexes and electrophoresis studies also showed greater activity for (R)-[VO(OMe)L] in cleaving DNA and protein as against (S)-[VO(OMe)L]. © 2011 Elsevier Masson SAS. All rights reserved.

Original language	American English
Pages (from-to)	5074-5085
Number of pages	12
Journal	European Journal of Medicinal Chemistry
Volume	46
Issue number	10
DOIs	https://doi.org/10.1016/j.ejmech.2011.08.020
State	Published - Oct 1 2011

Disciplines

Medical Specialties
Medicine and Health Sciences
Osteopathic Medicine and Osteopathy

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@article{1c21340ab8b44409af3fe27da379b96c,

title = "Influence of chirality of V(V) Schiff base complexes on DNA, BSA binding and cleavage activity.",

abstract = "New chiral V(V) Schiff base complexes (S)-[VO(OMe)L] and (R)-[VO(OMe)L] were synthesized and characterized by microanalysis, infrared (IR), UV-Visible, Circular dichroism (CD) spectroscopy and single crystal X-ray studies. The interaction of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA) protein showed chiral expression DNA/protein binding strength. The influence of chirality was also observed in cytotoxicity assay of Hep 2 cells. (R)-[VO(OMe)L] enantiomer exhibited higher binding constant (5 ± 1 × 10 5 M -1) as compared to (S)-[VO(OMe)L] (8 ± 1 × 10 4 M -1). The fluorescence quenching, thermal melting and viscosity data suggest DNA surface and/or groove binding nature of the complexes and electrophoresis studies also showed greater activity for (R)-[VO(OMe)L] in cleaving DNA and protein as against (S)-[VO(OMe)L]. {\textcopyright} 2011 Elsevier Masson SAS. All rights reserved.",

author = "Khan, \{N U\} and Naushira Pandya and \{Maity NCh\} and M Kumar and Patel, \{R M\} and Kureshy, \{R I\} and Abdi, \{S H\} and S Mishra and S Das and Bajaj, \{H C\}",

year = "2011",

month = oct,

day = "1",

doi = "10.1016/j.ejmech.2011.08.020",

language = "American English",

volume = "46",

pages = "5074--5085",

journal = "European Journal of Medicinal Chemistry",

issn = "0223-5234",

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TY - JOUR

T1 - Influence of chirality of V(V) Schiff base complexes on DNA, BSA binding and cleavage activity.

AU - Khan, N U

AU - Pandya, Naushira

AU - Maity NCh, null

AU - Kumar, M

AU - Patel, R M

AU - Kureshy, R I

AU - Abdi, S H

AU - Mishra, S

AU - Das, S

AU - Bajaj, H C

PY - 2011/10/1

Y1 - 2011/10/1

N2 - New chiral V(V) Schiff base complexes (S)-[VO(OMe)L] and (R)-[VO(OMe)L] were synthesized and characterized by microanalysis, infrared (IR), UV-Visible, Circular dichroism (CD) spectroscopy and single crystal X-ray studies. The interaction of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA) protein showed chiral expression DNA/protein binding strength. The influence of chirality was also observed in cytotoxicity assay of Hep 2 cells. (R)-[VO(OMe)L] enantiomer exhibited higher binding constant (5 ± 1 × 10 5 M -1) as compared to (S)-[VO(OMe)L] (8 ± 1 × 10 4 M -1). The fluorescence quenching, thermal melting and viscosity data suggest DNA surface and/or groove binding nature of the complexes and electrophoresis studies also showed greater activity for (R)-[VO(OMe)L] in cleaving DNA and protein as against (S)-[VO(OMe)L]. © 2011 Elsevier Masson SAS. All rights reserved.

AB - New chiral V(V) Schiff base complexes (S)-[VO(OMe)L] and (R)-[VO(OMe)L] were synthesized and characterized by microanalysis, infrared (IR), UV-Visible, Circular dichroism (CD) spectroscopy and single crystal X-ray studies. The interaction of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA) protein showed chiral expression DNA/protein binding strength. The influence of chirality was also observed in cytotoxicity assay of Hep 2 cells. (R)-[VO(OMe)L] enantiomer exhibited higher binding constant (5 ± 1 × 10 5 M -1) as compared to (S)-[VO(OMe)L] (8 ± 1 × 10 4 M -1). The fluorescence quenching, thermal melting and viscosity data suggest DNA surface and/or groove binding nature of the complexes and electrophoresis studies also showed greater activity for (R)-[VO(OMe)L] in cleaving DNA and protein as against (S)-[VO(OMe)L]. © 2011 Elsevier Masson SAS. All rights reserved.

UR - https://nsuworks.nova.edu/hpd_com_faculty_articles/1001

U2 - 10.1016/j.ejmech.2011.08.020

DO - 10.1016/j.ejmech.2011.08.020

M3 - Article

SN - 0223-5234

VL - 46

SP - 5074

EP - 5085

JO - European Journal of Medicinal Chemistry

JF - European Journal of Medicinal Chemistry

IS - 10

ER -

Influence of chirality of V(V) Schiff base complexes on DNA, BSA binding and cleavage activity.

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