KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

Karina Sampaio Caiaffa; Loiane Massunari; Marcelle Danelon; Gabriel Flores Abuna; Telma Blanca Lombardo Bedran; Norival Alves Santos-Filho; Denise Madalena Palomari Spolidorio; Natalia Leal Vizoto; Eduardo Maffud Cilli; Cristiane Duque

doi:10.1080/08927014.2017.1370087

KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

Karina Sampaio Caiaffa
, Loiane Massunari
, Marcelle Danelon
, Gabriel Flores Abuna
, Telma Blanca Lombardo Bedran
, Norival Alves Santos-Filho
, Denise Madalena Palomari Spolidorio
, Natalia Leal Vizoto
, Eduardo Maffud Cilli
, Cristiane Duque

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1C^V and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml⁻¹, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections.

Original language	English
Pages (from-to)	807-818
Number of pages	12
Journal	Biofouling
Volume	33
Issue number	10
DOIs	https://doi.org/10.1080/08927014.2017.1370087
State	Published - Nov 26 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.

ASJC Scopus Subject Areas

Aquatic Science
Applied Microbiology and Biotechnology
Water Science and Technology

Keywords

Biofilms
cationic antimicrobial peptides
cell culture
microbial sensitivity tests

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10.1080/08927014.2017.1370087

Cite this

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title = "KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens",

abstract = "This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections.",

keywords = "Biofilms, cationic antimicrobial peptides, cell culture, microbial sensitivity tests",

author = "Caiaffa, \{Karina Sampaio\} and Loiane Massunari and Marcelle Danelon and Abuna, \{Gabriel Flores\} and Bedran, \{Telma Blanca Lombardo\} and Santos-Filho, \{Norival Alves\} and Spolidorio, \{Denise Madalena Palomari\} and Vizoto, \{Natalia Leal\} and Cilli, \{Eduardo Maffud\} and Cristiane Duque",

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doi = "10.1080/08927014.2017.1370087",

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T1 - KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

AU - Caiaffa, Karina Sampaio

AU - Massunari, Loiane

AU - Danelon, Marcelle

AU - Abuna, Gabriel Flores

AU - Bedran, Telma Blanca Lombardo

AU - Santos-Filho, Norival Alves

AU - Spolidorio, Denise Madalena Palomari

AU - Vizoto, Natalia Leal

AU - Cilli, Eduardo Maffud

AU - Duque, Cristiane

PY - 2017/11/26

Y1 - 2017/11/26

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AB - This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections.

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KW - cationic antimicrobial peptides

KW - cell culture

KW - microbial sensitivity tests

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KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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