Methods for isolating the balbiani body/germplasm from xenopus laevis oocytes

Amanda Butler; Dawn Owens; Mary Lou King; Tristan Aguero

doi:10.1007/978-1-4939-9009-2_15

Methods for isolating the balbiani body/germplasm from xenopus laevis oocytes

Amanda Butler
, Dawn Owens
, Mary Lou King
, Tristan Aguero

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

The Balbiani body (Bb) is a large membrane-less organelle, densely packed with mitochondria, endoplasmic reticulum, proteins, and RNA. The Bb is present in many vertebrate female gametes. In frogs, the Bb is established early during oogenesis and operates as a maternal inherited embryonic determinant that specifies germline identity through the formation of germplasm. We describe here two techniques to isolate the Bb/germplasm from Xenopus laevis primary oocytes.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	265-275
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-9009-2_15
State	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1920
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Balbiani body-mitochondria cloud
Germ cells
Germplasm
Oogenesis
Xenopus

Access to Document

10.1007/978-1-4939-9009-2_15

Cite this

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title = "Methods for isolating the balbiani body/germplasm from xenopus laevis oocytes",

abstract = "The Balbiani body (Bb) is a large membrane-less organelle, densely packed with mitochondria, endoplasmic reticulum, proteins, and RNA. The Bb is present in many vertebrate female gametes. In frogs, the Bb is established early during oogenesis and operates as a maternal inherited embryonic determinant that specifies germline identity through the formation of germplasm. We describe here two techniques to isolate the Bb/germplasm from Xenopus laevis primary oocytes.",

keywords = "Balbiani body-mitochondria cloud, Germ cells, Germplasm, Oogenesis, Xenopus",

author = "Amanda Butler and Dawn Owens and King, \{Mary Lou\} and Tristan Aguero",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",

year = "2019",

doi = "10.1007/978-1-4939-9009-2\_15",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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TY - CHAP

T1 - Methods for isolating the balbiani body/germplasm from xenopus laevis oocytes

AU - Butler, Amanda

AU - Owens, Dawn

AU - King, Mary Lou

AU - Aguero, Tristan

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

N2 - The Balbiani body (Bb) is a large membrane-less organelle, densely packed with mitochondria, endoplasmic reticulum, proteins, and RNA. The Bb is present in many vertebrate female gametes. In frogs, the Bb is established early during oogenesis and operates as a maternal inherited embryonic determinant that specifies germline identity through the formation of germplasm. We describe here two techniques to isolate the Bb/germplasm from Xenopus laevis primary oocytes.

AB - The Balbiani body (Bb) is a large membrane-less organelle, densely packed with mitochondria, endoplasmic reticulum, proteins, and RNA. The Bb is present in many vertebrate female gametes. In frogs, the Bb is established early during oogenesis and operates as a maternal inherited embryonic determinant that specifies germline identity through the formation of germplasm. We describe here two techniques to isolate the Bb/germplasm from Xenopus laevis primary oocytes.

KW - Balbiani body-mitochondria cloud

KW - Germ cells

KW - Germplasm

KW - Oogenesis

KW - Xenopus

UR - https://www.scopus.com/pages/publications/85061278141

UR - https://www.scopus.com/pages/publications/85061278141#tab=citedBy

U2 - 10.1007/978-1-4939-9009-2_15

DO - 10.1007/978-1-4939-9009-2_15

M3 - Chapter

C2 - 30737696

AN - SCOPUS:85061278141

T3 - Methods in Molecular Biology

SP - 265

EP - 275

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Methods for isolating the balbiani body/germplasm from xenopus laevis oocytes

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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Analysis of actively transcribed DNA repair using a transfection-based system

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