Molecular evolution of glutamine synthetase II: Phylogenetic evidence of a non-endosymbiotic gene transfer event early in plant evolution

Background. Glutamine synthetase (GS) is essential for ammonium assimilation and the biosynthesis of glutamine. The three GS gene families (GSI, GSII, and GSIII) are represented in both prokaryotic and eukaryotic organisms. In this study, we examined the evolutionary relationship of GSII from eubacterial and eukaryotic lineages and present robust phylogenetic evidence that GSII was transferred from -Proteobacteria (Eubacteria) to the Chloroplastida. Results. GSII sequences were isolated from four species of green algae (Trebouxiophyceae), and additional green algal (Chlorophyceae and Prasinophytae) and streptophyte (Charales, Desmidiales, Bryophyta, Marchantiophyta, Lycopodiophyta and Tracheophyta) sequences were obtained from public databases. In Bayesian and maximum likelihood analyses, eubacterial (GSII _B) and eukaryotic (GSII _E) GSII sequences formed distinct clades. Both GSII _Band GSII _Ewere found in chlorophytes and early-diverging streptophytes. The GSII _Benzymes from these groups formed a well-supported sister clade with the -Proteobacteria, providing evidence that GSII _Bin the Chloroplastida arose by horizontal gene transfer (HGT). Bayesian relaxed molecular clock analyses suggest that GSII _Band GSII _E coexisted for an extended period of time but it is unclear whether the proposed HGT happened prior to or after the divergence of the primary endosymbiotic lineages (the Archaeplastida). However, GSII _Bgenes have not been identified in glaucophytes or red algae, favoring the hypothesis that GSII _Bwas gained after the divergence of the primary endosymbiotic lineages. Duplicate copies of the GSII _Bgene were present in Chlamydomonas reinhardtii, Volvox carteri f. nagariensis, and Physcomitrella patens. Both GSII _Bproteins in C. reinhardtii and V. carteri f. nagariensis had N-terminal transit sequences, indicating they are targeted to the chloroplast or mitochondrion. In contrast, GSII _Bproteins of P. patens lacked transit sequences, suggesting a cytosolic function. GSII _Bsequences were absent in vascular plants where the duplication of GSII _Ereplaced the function of GSII _B. Conclusions. Phylogenetic evidence suggests GSII _Bin Chloroplastida evolved by HGT, possibly after the divergence of the primary endosymbiotic lineages. Thus while multiple GS isoenzymes are common among members of the Chloroplastida, the isoenzymes may have evolved via different evolutionary processes. The acquisition of essential enzymes by HGT may provide rapid changes in biochemical capacity and therefore be favored by natural selection.