Monoclonal Antibodies to Conserved Regions of the Major Core Protein (gag24) of HIV-1 and HIV-2

Emilio Carpio; Carlos Duarte; Ana Campal; Jorge Gavilondo; Jorma Hinkula; Per Anders Broliden; Britta Wahren; Jon Roén; Mikael Jondal

doi:10.1089/aid.1991.7.97

Monoclonal Antibodies to Conserved Regions of the Major Core Protein (gag24) of HIV-1 and HIV-2

Emilio Carpio
, Carlos Duarte
, Ana Campal
, Jorge Gavilondo
, Jorma Hinkula
, Per Anders Broliden
, Britta Wahren
, Jon Roén
, Mikael Jondal

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Five mouse monoclonal antibodies were raised against a recombinant protein comprising the complete sequence of gag24 protein from HTLV-III_B. All monoclonal antibodies recognized the native protein in enzyme-linked immunosorbant assay (ELISA) and Western blots. All monoclonal antibodies also cross-reacted with an human immunodeficiency virus type 2 (HIV-2) strain in western blots, whereas only one antibody detected HIV-2 p25 in ELISA. By using synthetic peptides, cross-reacting epitopes were mapped and three regions were defined. The conserved immunogenic sites were located in the carboxyterminal region of the protein. Inhibition experiments with human sera showed that this region is also immunogenic in humans.

Original language	English
Pages (from-to)	97-101
Number of pages	5
Journal	AIDS Research and Human Retroviruses
Volume	7
Issue number	1
DOIs	https://doi.org/10.1089/aid.1991.7.97
State	Published - Jan 1991
Externally published	Yes

ASJC Scopus Subject Areas

Immunology
Virology
Infectious Diseases

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10.1089/aid.1991.7.97

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abstract = "Five mouse monoclonal antibodies were raised against a recombinant protein comprising the complete sequence of gag24 protein from HTLV-IIIB. All monoclonal antibodies recognized the native protein in enzyme-linked immunosorbant assay (ELISA) and Western blots. All monoclonal antibodies also cross-reacted with an human immunodeficiency virus type 2 (HIV-2) strain in western blots, whereas only one antibody detected HIV-2 p25 in ELISA. By using synthetic peptides, cross-reacting epitopes were mapped and three regions were defined. The conserved immunogenic sites were located in the carboxyterminal region of the protein. Inhibition experiments with human sera showed that this region is also immunogenic in humans.",

author = "Emilio Carpio and Carlos Duarte and Ana Campal and Jorge Gavilondo and Jorma Hinkula and Broliden, \{Per Anders\} and Britta Wahren and Jon Ro{\'e}n and Mikael Jondal",

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AU - Carpio, Emilio

AU - Duarte, Carlos

AU - Campal, Ana

AU - Gavilondo, Jorge

AU - Hinkula, Jorma

AU - Broliden, Per Anders

AU - Wahren, Britta

AU - Roén, Jon

AU - Jondal, Mikael

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AB - Five mouse monoclonal antibodies were raised against a recombinant protein comprising the complete sequence of gag24 protein from HTLV-IIIB. All monoclonal antibodies recognized the native protein in enzyme-linked immunosorbant assay (ELISA) and Western blots. All monoclonal antibodies also cross-reacted with an human immunodeficiency virus type 2 (HIV-2) strain in western blots, whereas only one antibody detected HIV-2 p25 in ELISA. By using synthetic peptides, cross-reacting epitopes were mapped and three regions were defined. The conserved immunogenic sites were located in the carboxyterminal region of the protein. Inhibition experiments with human sera showed that this region is also immunogenic in humans.

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Monoclonal Antibodies to Conserved Regions of the Major Core Protein (gag24) of HIV-1 and HIV-2

Abstract

ASJC Scopus Subject Areas

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