Oscillatory Shear Stress Created by Fluid Pulsatility versus Flexed Specimen Configurations

Manuel Salinas; David E. Schmidt; Miguel Libera; Richard R. Lange; Sharan Ramaswamy

doi:10.1080/10255842.2012.715157

Oscillatory Shear Stress Created by Fluid Pulsatility versus Flexed Specimen Configurations

Manuel Salinas
, David E. Schmidt
, Miguel Libera
, Richard R. Lange
, Sharan Ramaswamy

Research output: Contribution to journal › Article › peer-review

Abstract

Oscillatory shear stress (OSS), caused by time-varying flow environments, may play a critical role in the production of engineered tissue by bone marrow-derived stem cells. This is particularly relevant in heart valve tissue engineering (HVTE), owing to the intense haemodynamic environments that surround native valves. In this study, we examined and quantified the role that (i) physiologically relevant scales of pulsatility and (ii) changes in geometry as a function of specimen flexure have in creating OSS conditions. A U-shaped bioreactor capable of producing flow, stretch and flexure was modelled with housed specimens, and computational fluid dynamic simulations were performed. We found that physiologically relevant OSS can be maximised by the application of pulsatile flow to straight, non-moving specimens in a uniform manner. This finding reduces a substantial layer of complexity in dynamic HVTE protocols in which traditionally, time-varying flow has been promoted through specimen movement in custom-made bioreactors.

Original language	American English
Pages (from-to)	728-739
Number of pages	12
Journal	Computer Methods in Biomechanics and Biomedical Engineering
Volume	17
Issue number	7
DOIs	https://doi.org/10.1080/10255842.2012.715157
State	Published - Aug 24 2012

Funding

Funding for this work was provided by the American Heart Association (AHA), National Scientist Development Grant ID #: 0830061N (Sharan Ramaswamy). Graduate studies (Manuel Salinas) were supported through minority opportunities in biomedical research programmes – research initiative for scientific enhancement (MBRS-RISE) fellowship: NIH/NIGMS R25 GM061347. The authors acknowledge the Tecplot technical support centre for assistance with the usage of Tecplot 360 software. Finally, the authors thank Mr Jerry Centeno for his assistance with post-processing of data.

Funders	Funder number
NIH/NIGMS	R25 GM061347
National Scientist Development	0830061N
American Heart Association

ASJC Scopus Subject Areas

Bioengineering
Biomedical Engineering
Human-Computer Interaction
Computer Science Applications

Keywords

bone marrow stem cells
heart valve tissue engineering
oscillatory shear stress
pulsatile flow
time-varying flow

Disciplines

Computer Sciences

Access to Document

10.1080/10255842.2012.715157

Cite this

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title = "Oscillatory Shear Stress Created by Fluid Pulsatility versus Flexed Specimen Configurations",

abstract = " Oscillatory shear stress (OSS), caused by time-varying flow environments, may play a critical role in the production of engineered tissue by bone marrow-derived stem cells. This is particularly relevant in heart valve tissue engineering (HVTE), owing to the intense haemodynamic environments that surround native valves. In this study, we examined and quantified the role that (i) physiologically relevant scales of pulsatility and (ii) changes in geometry as a function of specimen flexure have in creating OSS conditions. A U-shaped bioreactor capable of producing flow, stretch and flexure was modelled with housed specimens, and computational fluid dynamic simulations were performed. We found that physiologically relevant OSS can be maximised by the application of pulsatile flow to straight, non-moving specimens in a uniform manner. This finding reduces a substantial layer of complexity in dynamic HVTE protocols in which traditionally, time-varying flow has been promoted through specimen movement in custom-made bioreactors.",

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AU - Salinas, Manuel

AU - Schmidt, David E.

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AU - Lange, Richard R.

AU - Ramaswamy, Sharan

PY - 2012/8/24

Y1 - 2012/8/24

N2 - Oscillatory shear stress (OSS), caused by time-varying flow environments, may play a critical role in the production of engineered tissue by bone marrow-derived stem cells. This is particularly relevant in heart valve tissue engineering (HVTE), owing to the intense haemodynamic environments that surround native valves. In this study, we examined and quantified the role that (i) physiologically relevant scales of pulsatility and (ii) changes in geometry as a function of specimen flexure have in creating OSS conditions. A U-shaped bioreactor capable of producing flow, stretch and flexure was modelled with housed specimens, and computational fluid dynamic simulations were performed. We found that physiologically relevant OSS can be maximised by the application of pulsatile flow to straight, non-moving specimens in a uniform manner. This finding reduces a substantial layer of complexity in dynamic HVTE protocols in which traditionally, time-varying flow has been promoted through specimen movement in custom-made bioreactors.

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KW - bone marrow stem cells

KW - heart valve tissue engineering

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UR - https://nsuworks.nova.edu/gscis_facarticles/366

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Oscillatory Shear Stress Created by Fluid Pulsatility versus Flexed Specimen Configurations

Abstract

Funding

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

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