Abstract
A one-step purification of mono-125I-angiotensin II prepared by the chloramine T procedure is described. The purification is effected on a cellulose cation exchange column with isocratic elution by 50 mM sodium acetate, pH 5.0. The purity of the mono-125I-angiotensin II was determined by thin layer chromatography, high pressure liquid chromatography, enzymatic digestion, radioreceptor assay, and radioimmunoassay. Preparation and purification of mono-125I-angiotensin II by this procedure offers significant advantages over existing methods for its preparation in terms of purity, simplicity, efficiency, and cost.
| Original language | English |
|---|---|
| Pages (from-to) | 137-150 |
| Number of pages | 14 |
| Journal | Journal of Pharmacological Methods |
| Volume | 11 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 1984 |
| Externally published | Yes |
ASJC Scopus Subject Areas
- Pharmacology
Keywords
- Angiotensin II
- Angiotensin II receptors
- Cation exchange chromatography
- mono-I-Angiotensin II
- Preparation
- Purification
Fingerprint
Dive into the research topics of 'Preparation and one-step purification of mono-125I-angiotensin II for radioligand binding assays'. Together they form a unique fingerprint.Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS