Primary cultures of human colon cancer as a model to study cancer stem cells

Sergey Koshkin; Anna Danilova; Grigory Raskin; Nikolai Petrov; Olga Bajenova; Stephen J. O’Brien; Alexey Tomilin; Elena Tolkunova; Stephen James O'Brien

doi:10.1007/s13277-016-5214-8

Primary cultures of human colon cancer as a model to study cancer stem cells

Sergey Koshkin
, Anna Danilova
, Grigory Raskin
, Nikolai Petrov
, Olga Bajenova
, Stephen J. O’Brien
, Alexey Tomilin
, Elena Tolkunova
, Stephen James O'Brien

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient’s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.

Original language	American English
Pages (from-to)	12833-12842
Number of pages	10
Journal	Tumor Biology
Volume	37
Issue number	9
DOIs	https://doi.org/10.1007/s13277-016-5214-8
State	Published - Sep 1 2016

Bibliographical note

Publisher Copyright:
© 2016, International Society of Oncology and BioMarkers (ISOBM).

Funding

This work was financially supported by the Russian Science Foundation (project 14-50-00068) to the Molecular Stem Cell Biology Laboratory, Institute of Cytology RAS, and by the Government of the Russian Federation mega grant 11G34.31.0068 to Dr. S. J. O'Brien.

Funders	Funder number
Institute of Cytology RAS
Molecular Stem Cell Biology Laboratory
Russian Science Foundation	14-50-00068
Government Council on Grants, Russian Federation	11G34.31.0068

ASJC Scopus Subject Areas

Cancer Research

Keywords

5-Fu
CD133
Cancer stem cells
Colon cancer
Drug resistance
Oct4

Disciplines

Genetics and Genomics
Life Sciences

Access to Document

10.1007/s13277-016-5214-8

https://doi.org/10.1007/s13277-016-5214-8

Cite this

@article{f353cccc0be3435ea53a62e5f6e95f3c,

title = "Primary cultures of human colon cancer as a model to study cancer stem cells",

abstract = " The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient{\textquoteright}s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.",

keywords = "5-Fu, CD133, Cancer stem cells, Colon cancer, Drug resistance, Oct4",

author = "Sergey Koshkin and Anna Danilova and Grigory Raskin and Nikolai Petrov and Olga Bajenova and O{\textquoteright}Brien, \{Stephen J.\} and Alexey Tomilin and Elena Tolkunova and O'Brien, \{Stephen James\}",

note = "Publisher Copyright: {\textcopyright} 2016, International Society of Oncology and BioMarkers (ISOBM).",

year = "2016",

month = sep,

day = "1",

doi = "10.1007/s13277-016-5214-8",

language = "American English",

volume = "37",

pages = "12833--12842",

journal = "Tumor Biology",

issn = "1010-4283",

publisher = "SAGE Publications Ltd",

number = "9",

}

TY - JOUR

T1 - Primary cultures of human colon cancer as a model to study cancer stem cells

AU - Koshkin, Sergey

AU - Danilova, Anna

AU - Raskin, Grigory

AU - Petrov, Nikolai

AU - Bajenova, Olga

AU - O’Brien, Stephen J.

AU - Tomilin, Alexey

AU - Tolkunova, Elena

AU - O'Brien, Stephen James

PY - 2016/9/1

Y1 - 2016/9/1

N2 - The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient’s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.

AB - The principal cause of death in cancer involves tumor progression and metastasis. Since only a small proportion of the primary tumor cells, cancer stem cells (CSCs), which are the most aggressive, have the capacity to metastasize and display properties of stem cells, it is imperative to characterize the gene expression of diagnostic markers and to evaluate the drug sensitivity in the CSCs themselves. Here, we have examined the key genes that are involved in the progression of colorectal cancer and are expressed in cancer stem cells. Primary cultures of colorectal cancer cells from a patient’s tumors were studied using the flow cytometry and cytological methods. We have evaluated the clinical and stem cell marker expression in these cells, their resistance to 5-fluorouracil and irinotecan, and the ability of cells to form tumors in mice. The data shows the role of stem cell marker Oct4 in the resistance of primary colorectal cancer tumor cells to 5-fluorouracil.

KW - 5-Fu

KW - CD133

KW - Cancer stem cells

KW - Colon cancer

KW - Drug resistance

KW - Oct4

UR - https://nsuworks.nova.edu/cnso_bio_facarticles/1104

UR - https://doi.org/10.1007/s13277-016-5214-8

UR - https://www.scopus.com/pages/publications/84979272998

UR - https://www.scopus.com/pages/publications/84979272998#tab=citedBy

U2 - 10.1007/s13277-016-5214-8

DO - 10.1007/s13277-016-5214-8

M3 - Article

SN - 1010-4283

VL - 37

SP - 12833

EP - 12842

JO - Tumor Biology

JF - Tumor Biology

IS - 9

ER -

Primary cultures of human colon cancer as a model to study cancer stem cells

Abstract

Bibliographical note

Funding

ASJC Scopus Subject Areas

Keywords

Disciplines

Access to Document

Other files and links

Fingerprint

Cite this