Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine

Myreisa Morales-Cruz; Marjorie López-Nieves; Roberto Morales-Hernández; Gian C. Rivera-Crespo; Gary A. Toranzos; Ileana González-González; Carlos R. Cabrera

doi:10.1016/j.bioelechem.2018.03.017

Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine

Myreisa Morales-Cruz
, Marjorie López-Nieves
, Roberto Morales-Hernández
, Gian C. Rivera-Crespo
, Gary A. Toranzos
, Ileana González-González
, Carlos R. Cabrera

Research output: Contribution to journal › Article › peer-review

Abstract

One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5 × 10⁹ cfu/mL Proteus vulgaris in synthetic urine.

Original language	English
Pages (from-to)	206-212
Number of pages	7
Journal	Bioelectrochemistry
Volume	122
DOIs	https://doi.org/10.1016/j.bioelechem.2018.03.017
State	Published - Aug 2018
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018

ASJC Scopus Subject Areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

Keywords

Ammonia oxidation reaction
Proteus vulgaris
Urea
Ureolysis
Urine

Access to Document

10.1016/j.bioelechem.2018.03.017

Cite this

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title = "Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine",

abstract = "One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in \textasciitilde{}2.5 × 109 cfu/mL Proteus vulgaris in synthetic urine.",

keywords = "Ammonia oxidation reaction, Proteus vulgaris, Urea, Ureolysis, Urine",

author = "Myreisa Morales-Cruz and Marjorie L{\'o}pez-Nieves and Roberto Morales-Hern{\'a}ndez and Rivera-Crespo, \{Gian C.\} and Toranzos, \{Gary A.\} and Ileana Gonz{\'a}lez-Gonz{\'a}lez and Cabrera, \{Carlos R.\}",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = aug,

doi = "10.1016/j.bioelechem.2018.03.017",

language = "English",

volume = "122",

pages = "206--212",

journal = "Bioelectrochemistry",

issn = "1567-5394",

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TY - JOUR

T1 - Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine

AU - Morales-Cruz, Myreisa

AU - López-Nieves, Marjorie

AU - Morales-Hernández, Roberto

AU - Rivera-Crespo, Gian C.

AU - Toranzos, Gary A.

AU - González-González, Ileana

AU - Cabrera, Carlos R.

PY - 2018/8

Y1 - 2018/8

N2 - One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5 × 109 cfu/mL Proteus vulgaris in synthetic urine.

AB - One of the most challenging problems when trying to recycle urine for different purposes is the removal of urea. In this project we studied an ureolysis system using the bacterium Proteus vulgaris for the transformation of urea to ammonia and its subsequent oxidation to nitrogen at a Pt working electrode. Our system was tested under different pH, microbial reaction times, and urea and bacteria concentrations. Our results indicate that a pH 8 is optimal for the combined Proteus vulgaris urease activity and the ammonia oxidation reaction at a Pt electrode. The reaction time and concentration dependence on the ammonia oxidation reaction current densities was also studied. Results showed limited ammonia oxidation under high urea concentrations in ~2.5 × 109 cfu/mL Proteus vulgaris in synthetic urine.

KW - Ammonia oxidation reaction

KW - Proteus vulgaris

KW - Urea

KW - Ureolysis

KW - Urine

UR - https://www.scopus.com/pages/publications/85045709308

UR - https://www.scopus.com/pages/publications/85045709308#tab=citedBy

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DO - 10.1016/j.bioelechem.2018.03.017

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AN - SCOPUS:85045709308

SN - 1567-5394

VL - 122

SP - 206

EP - 212

JO - Bioelectrochemistry

JF - Bioelectrochemistry

ER -

Proteus vulgaris - Pt electrode system for urea to nitrogen conversion in synthetic urine

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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Cite this