Radiolabeling of umbilical cord-derived mesenchymal stem cells for in vivo tracking

Archana Mukherjee; Shabari Tipnis; Haladhar Dev Sarma; Geeta Ravindran; Grace Samuel; Chandra Viswanathan; Meera Venkatesh

doi:10.1089/cbr.2011.1146

Radiolabeling of umbilical cord-derived mesenchymal stem cells for in vivo tracking

Archana Mukherjee
, Shabari Tipnis
, Haladhar Dev Sarma
, Geeta Ravindran
, Grace Samuel
, Chandra Viswanathan
, Meera Venkatesh

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Non-invasive methods for the assessment of distribution, homing, and retention of stem cells are desired for the successful demonstration of stem cell therapy. Cells labeled with ^99mTc, ¹⁸F, and ¹¹¹In have been reported for tracking the cells in vivo. However, they can be tracked only for a limited time due to the short half lives of these isotopes. In this context, stem cells labeled with ⁵¹Cr would be appropriate for tracking cells for a longer period of time owing to their half life of 27.7 days. Here, we have isolated mesenchymal stem cells (MSCs) from umbilical cord tissue, characterized them, and attempted to radiolabel them with ⁵¹Cr for mapping the fate of transplanted MSC cells after an intravenous injection via the tail vein in small animals.

Original language	English
Pages (from-to)	614-619
Number of pages	6
Journal	Cancer Biotherapy and Radiopharmaceuticals
Volume	27
Issue number	9
DOIs	https://doi.org/10.1089/cbr.2011.1146
State	Published - Nov 1 2012
Externally published	Yes

ASJC Scopus Subject Areas

Oncology
Radiology Nuclear Medicine and imaging
Pharmacology
Cancer Research

Keywords

Cr-labeled stem cells
Radiolabeled stem cells
Umbilical cord mesenchymal stem cells (UCMSC)

Access to Document

10.1089/cbr.2011.1146

Cite this

@article{c6c98fe9a09645d5be9a9c4629f05efb,

title = "Radiolabeling of umbilical cord-derived mesenchymal stem cells for in vivo tracking",

abstract = "Non-invasive methods for the assessment of distribution, homing, and retention of stem cells are desired for the successful demonstration of stem cell therapy. Cells labeled with 99mTc, 18F, and 111In have been reported for tracking the cells in vivo. However, they can be tracked only for a limited time due to the short half lives of these isotopes. In this context, stem cells labeled with 51Cr would be appropriate for tracking cells for a longer period of time owing to their half life of 27.7 days. Here, we have isolated mesenchymal stem cells (MSCs) from umbilical cord tissue, characterized them, and attempted to radiolabel them with 51Cr for mapping the fate of transplanted MSC cells after an intravenous injection via the tail vein in small animals.",

keywords = "Cr-labeled stem cells, Radiolabeled stem cells, Umbilical cord mesenchymal stem cells (UCMSC)",

author = "Archana Mukherjee and Shabari Tipnis and Sarma, \{Haladhar Dev\} and Geeta Ravindran and Grace Samuel and Chandra Viswanathan and Meera Venkatesh",

year = "2012",

month = nov,

day = "1",

doi = "10.1089/cbr.2011.1146",

language = "English",

volume = "27",

pages = "614--619",

journal = "Cancer Biotherapy and Radiopharmaceuticals",

issn = "1084-9785",

publisher = "Mary Ann Liebert Inc.",

number = "9",

}

TY - JOUR

T1 - Radiolabeling of umbilical cord-derived mesenchymal stem cells for in vivo tracking

AU - Mukherjee, Archana

AU - Tipnis, Shabari

AU - Sarma, Haladhar Dev

AU - Ravindran, Geeta

AU - Samuel, Grace

AU - Viswanathan, Chandra

AU - Venkatesh, Meera

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Non-invasive methods for the assessment of distribution, homing, and retention of stem cells are desired for the successful demonstration of stem cell therapy. Cells labeled with 99mTc, 18F, and 111In have been reported for tracking the cells in vivo. However, they can be tracked only for a limited time due to the short half lives of these isotopes. In this context, stem cells labeled with 51Cr would be appropriate for tracking cells for a longer period of time owing to their half life of 27.7 days. Here, we have isolated mesenchymal stem cells (MSCs) from umbilical cord tissue, characterized them, and attempted to radiolabel them with 51Cr for mapping the fate of transplanted MSC cells after an intravenous injection via the tail vein in small animals.

AB - Non-invasive methods for the assessment of distribution, homing, and retention of stem cells are desired for the successful demonstration of stem cell therapy. Cells labeled with 99mTc, 18F, and 111In have been reported for tracking the cells in vivo. However, they can be tracked only for a limited time due to the short half lives of these isotopes. In this context, stem cells labeled with 51Cr would be appropriate for tracking cells for a longer period of time owing to their half life of 27.7 days. Here, we have isolated mesenchymal stem cells (MSCs) from umbilical cord tissue, characterized them, and attempted to radiolabel them with 51Cr for mapping the fate of transplanted MSC cells after an intravenous injection via the tail vein in small animals.

KW - Cr-labeled stem cells

KW - Radiolabeled stem cells

KW - Umbilical cord mesenchymal stem cells (UCMSC)

UR - https://www.scopus.com/pages/publications/84868286832

UR - https://www.scopus.com/pages/publications/84868286832#tab=citedBy

U2 - 10.1089/cbr.2011.1146

DO - 10.1089/cbr.2011.1146

M3 - Article

C2 - 23046460

AN - SCOPUS:84868286832

SN - 1084-9785

VL - 27

SP - 614

EP - 619

JO - Cancer Biotherapy and Radiopharmaceuticals

JF - Cancer Biotherapy and Radiopharmaceuticals

IS - 9

ER -

Radiolabeling of umbilical cord-derived mesenchymal stem cells for in vivo tracking

Abstract

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Fingerprint

Cite this