Rotifer Micro-Anatomy as Revealed by Phallacidin and Propidium Iodide Staining and Confocal Microscopy

Research output: Contribution to conferencePresentation

Abstract

Rotifers (Brachionus plicatilis) were fixed and stained with BODIPY-FL Phallacidin, a filamentous actin stain (muscle fibers) and Propidium Iodide, a DNA stain. Visualization with an ultraviolet light source and a confocal microscope revealed fine scale three-dimensional morphology of feeding structures, muscle tissues, syncytial tissues and reproductive organs. This poster presents three-dimensional images specifically showing the mastax and details of its muscular complex, the syncytial vitellarium at various stages of embryogenesis and the coronal and pedal retractor muscles. These images allow for future comparisons of functional morphology with other rotifer species of different diets and habitats.

Original languageAmerican English
StatePublished - Jan 7 2004
Externally publishedYes
Event2004 Society for Integrative and Comparative Biology Meeting - New Orleans, United States
Duration: Jan 5 2004Jan 9 2004

Conference

Conference2004 Society for Integrative and Comparative Biology Meeting
Country/TerritoryUnited States
CityNew Orleans
Period1/5/041/9/04

Disciplines

  • Marine Biology
  • Oceanography and Atmospheric Sciences and Meteorology

Fingerprint

Dive into the research topics of 'Rotifer Micro-Anatomy as Revealed by Phallacidin and Propidium Iodide Staining and Confocal Microscopy'. Together they form a unique fingerprint.

Cite this