Secretion in mast cells induced by calcium entrapped within phospholipid vesicles

Theoharis C. Theoharides; William W. Douglas

doi:10.1126/science.684435

Secretion in mast cells induced by calcium entrapped within phospholipid vesicles

Theoharis C. Theoharides
, William W. Douglas

Research output: Contribution to journal › Article › peer-review

Abstract

Purified mast cells secreted histamine when fused to phospholipid vesicles containing calcium but not magnesium or potassium. Microscopic observation revealed highly localized exocytotic responses involving punctate extrusion of individual granules. Calcium delivered from the vesicles to the cytoplasm is apparently a sufficient stimulus to initiate exocytosis. The results support the calcium hypothesis of stimulus-secretion coupling.

Original language	English
Pages (from-to)	1143-1144
Number of pages	2
Journal	Science
Volume	201
Issue number	4361
DOIs	https://doi.org/10.1126/science.684435
State	Published - 1978
Externally published	Yes

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title = "Secretion in mast cells induced by calcium entrapped within phospholipid vesicles",

abstract = "Purified mast cells secreted histamine when fused to phospholipid vesicles containing calcium but not magnesium or potassium. Microscopic observation revealed highly localized exocytotic responses involving punctate extrusion of individual granules. Calcium delivered from the vesicles to the cytoplasm is apparently a sufficient stimulus to initiate exocytosis. The results support the calcium hypothesis of stimulus-secretion coupling.",

author = "Theoharides, \{Theoharis C.\} and Douglas, \{William W.\}",

year = "1978",

doi = "10.1126/science.684435",

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AU - Theoharides, Theoharis C.

AU - Douglas, William W.

PY - 1978

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AB - Purified mast cells secreted histamine when fused to phospholipid vesicles containing calcium but not magnesium or potassium. Microscopic observation revealed highly localized exocytotic responses involving punctate extrusion of individual granules. Calcium delivered from the vesicles to the cytoplasm is apparently a sufficient stimulus to initiate exocytosis. The results support the calcium hypothesis of stimulus-secretion coupling.

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U2 - 10.1126/science.684435

DO - 10.1126/science.684435

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AN - SCOPUS:0018188706

SN - 0036-8075

VL - 201

SP - 1143

EP - 1144

JO - Science

JF - Science

IS - 4361

ER -

Secretion in mast cells induced by calcium entrapped within phospholipid vesicles

Abstract

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