Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis

Jackson Gamer; Derek Van Booven; Oskar Zarnowski; Melanie Perez; Joshua Frank; Rajendra P. Pangeni; Fanny Collado; Nancy G. Klimas; Elisa Oltra; Lubov Nathanson

doi:10.1007/978-1-0716-4498-0_7

Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis

Jackson Gamer
, Derek Van Booven
, Oskar Zarnowski
, Melanie Perez
, Joshua Frank
, Rajendra P. Pangeni
, Fanny Collado
, Nancy G. Klimas
, Elisa Oltra
, Lubov Nathanson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic, complex multi-organ illness characterized by unexplained debilitating fatigue and post-exertional malaise (PEM). We evaluated transcriptomic changes in peripheral blood mononuclear cells (PBMC) of ME/CFS patients undergoing an exercise challenge and explored the transcriptomic response to exercise and recovery in PBMC of ME/CFS patients, as compared to healthy controls using RNA sequencing technology. As transcriptomic changes in ME/CFS patients are still in the phase of discovery, analysis of data has to be stringent, and the most important results have to be validated by a different technology, such as real-time PCR or NanoString.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	103-112
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-0716-4498-0_7
State	Published - 2025

Publication series

Name	Methods in Molecular Biology
Volume	2920
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)
Peripheral blood mononuclear cells (PBMC)
RNA extraction
RNA-seq data analysis
Transcriptomics

Access to Document

10.1007/978-1-0716-4498-0_7

Cite this

Gamer, J., Van Booven, D., Zarnowski, O., Perez, M., Frank, J., Pangeni, R. P., Collado, F., Klimas, N. G., Oltra, E., & Nathanson, L. (2025). Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis. In Methods in Molecular Biology (pp. 103-112). (Methods in Molecular Biology; Vol. 2920). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-4498-0_7

Gamer, J, Van Booven, D, Zarnowski, O, Perez, M, Frank, J, Pangeni, RP , Collado, F , Klimas, NG, Oltra, E & Nathanson, L 2025, Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2920, Humana Press Inc., pp. 103-112. https://doi.org/10.1007/978-1-0716-4498-0_7

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abstract = "Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic, complex multi-organ illness characterized by unexplained debilitating fatigue and post-exertional malaise (PEM). We evaluated transcriptomic changes in peripheral blood mononuclear cells (PBMC) of ME/CFS patients undergoing an exercise challenge and explored the transcriptomic response to exercise and recovery in PBMC of ME/CFS patients, as compared to healthy controls using RNA sequencing technology. As transcriptomic changes in ME/CFS patients are still in the phase of discovery, analysis of data has to be stringent, and the most important results have to be validated by a different technology, such as real-time PCR or NanoString.",

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AU - Frank, Joshua

AU - Pangeni, Rajendra P.

AU - Collado, Fanny

AU - Klimas, Nancy G.

AU - Oltra, Elisa

AU - Nathanson, Lubov

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Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis

Abstract

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ASJC Scopus Subject Areas

Keywords

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