The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]

Jose Victor Lopez; Shirley A. Pomponi; Ute Hentschel; Dirk Erpenbeck; Nina Pruzinsky; Cara Fiore; Rebecca Mulheron; Graeme Oatley; Elizabeth Sinclair; Eerik Aunin; Noah Gettle; Camilla Santos; Michael Paulini; Haoyu Niu; Victoria McKenna; Rebecca O’Brien; Samples and Laboratory Team Wellcome Sanger Institute Tree of Life Management; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Wellcome Sanger Institute Tree of Life Core Informatics Team; EBI Aquatic Symbiosis Genomics Data Portal Team; Aquatic Symbiosis Genomics Project Leadership

doi:10.12688/wellcomeopenres.24173.2

The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]

Jose Victor Lopez
, Shirley A. Pomponi
, Ute Hentschel
, Dirk Erpenbeck
, Nina Pruzinsky
, Cara Fiore
, Rebecca Mulheron
, Graeme Oatley
, Elizabeth Sinclair
, Eerik Aunin
, Noah Gettle
, Camilla Santos
, Michael Paulini
, Haoyu Niu
, Victoria McKenna
, Rebecca O’Brien
, Samples and Laboratory Team Wellcome Sanger Institute Tree of Life Management
, Wellcome Sanger Institute Scientific Operations: Sequencing Operations
, Wellcome Sanger Institute Tree of Life Core Informatics Team
, EBI Aquatic Symbiosis Genomics Data Portal Team

Aquatic Symbiosis Genomics Project Leadership

Research output: Contribution to journal › Article › peer-review

Abstract

We present a genome assembly from a specimen of Xestospongia muta (Caribbean barrel sponge; Porifera; Demospongiae; Haplosclerida; Petrosiidae). The genome sequence has a total length of 158.52 megabases. Most of the assembly (99.56%) is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 18.99 kilobases in length. Several symbiotic bacterial genomes were assembled as MAGs, including Candidatus Poribacteria species, Candidatus Latescibacteria, Acidobacteriota, Actinomycetota Gemmatimonadota, multiple Chloroflexota and the archaeon Nitrosopumilus. Gene annotation of this assembly on Ensembl identified 20,220 protein-coding genes.

Original language	English
Journal	Wellcome Open Research
DOIs	https://doi.org/10.12688/wellcomeopenres.24173.2
State	Submitted - Oct 1 2025

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10.12688/wellcomeopenres.24173.2

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Lopez, J. V., Pomponi, S. A., Hentschel, U., Erpenbeck, D., Pruzinsky, N., Fiore, C., Mulheron, R., Oatley, G., Sinclair, E., Aunin, E., Gettle, N., Santos, C., Paulini, M., Niu, H., McKenna, V., O’Brien, R., Wellcome Sanger Institute Tree of Life Management, S. A. L. T., Operations, W. S. I. S. O. S., Team, W. S. I. T. O. L. C. I., ... Leadership, A. S. G. P. (2025). The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]. Manuscript submitted for publication. https://doi.org/10.12688/wellcomeopenres.24173.2

Lopez, JV, Pomponi, SA, Hentschel, U, Erpenbeck, D, Pruzinsky, N, Fiore, C, Mulheron, R, Oatley, G, Sinclair, E, Aunin, E, Gettle, N, Santos, C, Paulini, M, Niu, H, McKenna, V, O’Brien, R, Wellcome Sanger Institute Tree of Life Management, SALT, Operations, WSISOS, Team, WSITOLCI, Team, EBIASGDP & Leadership, ASGP 2025, 'The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]', Wellcome Open Research. https://doi.org/10.12688/wellcomeopenres.24173.2

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title = "The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]",

abstract = "We present a genome assembly from a specimen of Xestospongia muta (Caribbean barrel sponge; Porifera; Demospongiae; Haplosclerida; Petrosiidae). The genome sequence has a total length of 158.52 megabases. Most of the assembly (99.56\%) is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 18.99 kilobases in length. Several symbiotic bacterial genomes were assembled as MAGs, including Candidatus Poribacteria species, Candidatus Latescibacteria, Acidobacteriota, Actinomycetota Gemmatimonadota, multiple Chloroflexota and the archaeon Nitrosopumilus. Gene annotation of this assembly on Ensembl identified 20,220 protein-coding genes.",

author = "Lopez, \{Jose Victor\} and Pomponi, \{Shirley A.\} and Ute Hentschel and Dirk Erpenbeck and Nina Pruzinsky and Cara Fiore and Rebecca Mulheron and Graeme Oatley and Elizabeth Sinclair and Eerik Aunin and Noah Gettle and Camilla Santos and Michael Paulini and Haoyu Niu and Victoria McKenna and Rebecca O{\textquoteright}Brien and \{Wellcome Sanger Institute Tree of Life Management\}, \{Samples and Laboratory Team\} and Operations, \{Wellcome Sanger Institute Scientific Operations: Sequencing\} and Team, \{Wellcome Sanger Institute Tree of Life Core Informatics\} and Team, \{EBI Aquatic Symbiosis Genomics Data Portal\} and Leadership, \{Aquatic Symbiosis Genomics Project\}",

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doi = "10.12688/wellcomeopenres.24173.2",

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T1 - The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]

AU - Lopez, Jose Victor

AU - Pomponi, Shirley A.

AU - Hentschel, Ute

AU - Erpenbeck, Dirk

AU - Pruzinsky, Nina

AU - Fiore, Cara

AU - Mulheron, Rebecca

AU - Oatley, Graeme

AU - Sinclair, Elizabeth

AU - Aunin, Eerik

AU - Gettle, Noah

AU - Santos, Camilla

AU - Paulini, Michael

AU - Niu, Haoyu

AU - McKenna, Victoria

AU - O’Brien, Rebecca

AU - Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory Team

AU - Operations, Wellcome Sanger Institute Scientific Operations: Sequencing

AU - Team, Wellcome Sanger Institute Tree of Life Core Informatics

AU - Team, EBI Aquatic Symbiosis Genomics Data Portal

AU - Leadership, Aquatic Symbiosis Genomics Project

PY - 2025/10/1

Y1 - 2025/10/1

N2 - We present a genome assembly from a specimen of Xestospongia muta (Caribbean barrel sponge; Porifera; Demospongiae; Haplosclerida; Petrosiidae). The genome sequence has a total length of 158.52 megabases. Most of the assembly (99.56%) is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 18.99 kilobases in length. Several symbiotic bacterial genomes were assembled as MAGs, including Candidatus Poribacteria species, Candidatus Latescibacteria, Acidobacteriota, Actinomycetota Gemmatimonadota, multiple Chloroflexota and the archaeon Nitrosopumilus. Gene annotation of this assembly on Ensembl identified 20,220 protein-coding genes.

AB - We present a genome assembly from a specimen of Xestospongia muta (Caribbean barrel sponge; Porifera; Demospongiae; Haplosclerida; Petrosiidae). The genome sequence has a total length of 158.52 megabases. Most of the assembly (99.56%) is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 18.99 kilobases in length. Several symbiotic bacterial genomes were assembled as MAGs, including Candidatus Poribacteria species, Candidatus Latescibacteria, Acidobacteriota, Actinomycetota Gemmatimonadota, multiple Chloroflexota and the archaeon Nitrosopumilus. Gene annotation of this assembly on Ensembl identified 20,220 protein-coding genes.

UR - https://wellcomeopenresearch.org/articles/10-336/v2

U2 - 10.12688/wellcomeopenres.24173.2

DO - 10.12688/wellcomeopenres.24173.2

M3 - Article

SN - 2398-502X

JO - Wellcome Open Research

JF - Wellcome Open Research

ER -

The chromosomal genome sequence of the giant barrel sponge, Xestospongia muta Schmidt 1870 and its associated microbial metagenome sequences [version 2; peer review: 2 approved]

Abstract

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